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3'-Acetyl-1'-benzyl-2'-methylpyrrolo[3,2-C]4-deoxyrifamycin
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3-(4-Benzyl-2,6-dimethyl piperazinoiminomethyl)rifamycin SV
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3-(4-Ethylpiperazinoiminomethyl) rifamycin SV
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5-epi-sisomycin
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pH-dependent inhibition, non-competitive inhibitor for ATP
8-amino-ATP
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C-8 substitution influences sugar pucker conformation, which may affect yPAP efficiently
8-aza-ATP
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slight inhibition
8-azido-ATP
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slight inhibition
8-bromo-ATP
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halogen modification at C-8 may negatively affect the ability of the enzyme to activate the ATP substrate or to transfer the AMP group from the enzyme to the RNA substrate
adenosine 5'-(alpha,beta-methylenetriphosphate)
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adenylyl-(3'-5')adenosine
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Adenylyl-(3'-5')cytosine
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ADP
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inhibits hydrolytic reaction
alpha, beta-methylene-ATP
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a nonreactive ATP analogue
AMP
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inhibits hydrolytic reaction
aurintricarboxylic acid
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CaCl2
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remaining activity 3%
CoCl2
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remaining activity 75%
Cordycepin 5'-triphosphate
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Cordycepin triphosphate
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5.0 mM
CuCl2
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remaining activity 0.1%
EDTA
100% inhibition at 0.5 mM
GMP
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1 mM, complete inhibition of enzymic reaction with tRNA
hygromycin B
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pH-dependent inhibition
kanamycin A
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pH-dependent inhibition
kanamycin B
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pH-dependent inhibition
KCl
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stimulation at low concentrations, at 300 mM, 15% inhibition, major poly(A) polymerase, 52% inhibition, minor poly(A) polymerase
lividomycin A
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pH-dependent inhibition
Mn2+
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high concentrations
NaF
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10 mM, complete inhibition
neomycin B
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an increase in pH releases the neomycin B inhibitory effect on PAPgamma
Pancreatic ribonuclease
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paromomycin
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pH-dependent inhibition
PD98059
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induces partial inhibition of PAP phosphorylation at S537
Ribonucleoside triphosphates other than ATP
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ribostamycin
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pH-dependent inhibition
Rifamycin B:N,N-diethylamide
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Rifamycin B:N,N-dipentylamide
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Rifamycin derivatives
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some derivatives are effective, others not
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sisomicin
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pH-dependent inhibition, non-competitive inhibitor for ATP
small ubiquitin-like modifier
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in vitro sumoylation inhibits the activity of purified PAP
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tobramycin
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pH-dependent inhibition
1,10-phenanthroline
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1 mM, 28% inhibition, major poly(A) polymerase, 50% inhibition, minor poly(A) polymerase
2'-dATP
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3'-dATP
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ATP
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above 0.5 mM
ATP
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hepatoma enzyme less effective to substrate inhibition than liver enzyme
ATP
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inhibits hydrolytic reaction
cordycepin
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not
CTP
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dATP
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dATP
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0.25 mM, 50% inhibition, major poly(A) polymerase, 15% inhibition, minor poly(A) polymerase
diphosphate
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noncompetitive to ATP and primer
diphosphate
Coturnix sp.
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noncompetitive to ATP and primer
diphosphate
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noncompetitive to ATP and primer
diphosphate
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noncompetitive to ATP and primer
diphosphate
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noncompetitive to ATP and primer
diphosphate
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noncompetitive to ATP and primer
diphosphate
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noncompetitive to ATP and primer
diphosphate
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product inhibitor
diphosphate
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noncompetitive to ATP and primer
GTP
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K+
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K+
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80 mM: 50% inhibition; KCl
K+
Coturnix sp.
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above 50 mM; KCl
K+
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KCl; maximal stimulation at 40 mM, inhibition above 250 mM
N-ethylmaleimide
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inhibits Mn2+-activated enzyme
N-ethylmaleimide
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inhibits Mn2+-activated enzyme
Na+
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Na+
Coturnix sp.
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above 50 mM; NaCl
Na2HPO4
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Na2HPO4
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dibasic sodium phosphate
NH4+
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NH4+
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50 mM: 50% inhibition; ammonium sulfate
NH4+
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10-40 mM; ammonium sulfate
NH4+
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ammonium sulfate; maximal activity at 33 mM, inhibition above 150 mM
NH4+
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0.1 M polymerase Ia and Ib completely inhibited, polymerase II: 68% inhibition; ammonium sulfate
phosphate
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phosphate
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not: rat liver nuclear enzyme
PO43-
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not inhibitory
Poly(dT)
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polyamines
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polyamines
Coturnix sp.
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Polyphosphate
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with 20 nM to 0.002 mM of polyphosphate the length of the products is reduced but inhibition is not complete
Polyphosphate
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inhibition depends on chain length, P3 10% at 0.001 mM, P4 60% at 0.001 mM, P15 90% at 0.001 mM, P35 100% at 0.001 mM
Polyphosphate
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potent inhibitor of poly(A) polymerase activity, almost complete inhibition at 200 nM
Proflavine
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only at very high levels
Proflavine
Coturnix sp.
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only at very high levels
Proflavine
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only at very high levels
Proflavine
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only at very high levels
Proflavine
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only at very high levels
Proflavine
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only at very high levels
Proflavine
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only at very high levels
Proflavine
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only at very high levels
rifampicin
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not inhibitory
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
Coturnix sp.
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
rifamycin AF/013
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O-n-octyloxime of 3-formylrifamycin SV
SO42-
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spermine
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inhibition if poly(A), nuclear RNA, or tRNA serves as primer, not with short oligonucleotide primers such as (Ap)3A
spermine
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inhibition if poly(A), nuclear RNA, or tRNA serves as primer, not with short oligonucleotide primers such as (Ap)3A
UTP
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Zn2+
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Zn2+
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inhibits PAP activity at concentrations above 10 ยตM in the presence of 5 mM MgCl2
additional information
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not inhibitory: rifampicin, streptolydigin, phosphate at 0.5 mM, cordycepin at 0.1 mM
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additional information
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overview
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additional information
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not inhibitory: actinomycin D; not inhibitory: alpha-amanitin
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additional information
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insensitive to high levels of RNA-polymerase inhibitors
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additional information
Coturnix sp.
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overview
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additional information
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overview
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additional information
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overview
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additional information
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stem-loop structure in mRNA 3ย-ends may be inhibitory
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additional information
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overview
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additional information
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not inhibited by neamine
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additional information
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not inhibitory: alpha-amanitin
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additional information
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overview
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additional information
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overview
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additional information
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not inhibitory: alpha-amanitin
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additional information
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not inhibitory: 3-formalrifamycin SV:o-methyloxime; not inhibitory: 4-(dimethylamino)-4-deoxyrifamycin SV
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additional information
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Fip1, a component of yeast polyadenylation factor I, has an inhibitory effect on the enzyme activity because it competes with RNA for access to the C-RBS region
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additional information
Fippbd peptide does not inhibit Pap1 activity even at concentrations in excess of 0.2 mM
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additional information
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Fippbd peptide does not inhibit Pap1 activity even at concentrations in excess of 0.2 mM
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additional information
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not inhibitory: alpha-amanitin
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additional information
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overview
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additional information
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cytoplasmic polyadenylation elements and PUF-binding elements are required for repression of GLD-2 mRNA
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