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Literature summary for 2.7.7.19 extracted from

  • Sarkar, B.; Cao, G.J.; Sarkar, N.
    Identification of two poly(A) polymerases in Bacillus subtilis (1997), Biochem. Mol. Biol. Int., 41, 1045-1050.
    View publication on PubMed
Search for more literature for 2.7.7.19

Activating Compound

Activating Compound Comment Organism Structure
KCl major poly(A) polymerase, optimal at 250 mM, minor poly(A) polymerase, optimal at 125 mM, inhibition of both above 250 mM Bacillus subtilis
spermidine slight stimulation, 5 mM Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 28% inhibition, major poly(A) polymerase, 50% inhibition, minor poly(A) polymerase Bacillus subtilis
cordycepin not Bacillus subtilis
CTP
-
 Bacillus subtilis
dATP 0.25 mM, 50% inhibition, major poly(A) polymerase, 15% inhibition, minor poly(A) polymerase Bacillus subtilis
GTP
-
 Bacillus subtilis
KCl stimulation at low concentrations, at 300 mM, 15% inhibition, major poly(A) polymerase, 52% inhibition, minor poly(A) polymerase Bacillus subtilis
additional information not inhibitory: rifampicin, streptolydigin, phosphate at 0.5 mM, cordycepin at 0.1 mM Bacillus subtilis
PO43- not inhibitory Bacillus subtilis
rifampicin not inhibitory Bacillus subtilis
UTP
-
 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.143
-
 ATP major poly(A) polymerase, 37°C, pH 8.0 Bacillus subtilis
0.4
-
 ATP major poly(A) polymerase, 37°C, pH 8.0 Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Bacillus subtilis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ more active in presence of Mn2+ than Mg2+ Bacillus subtilis
Mn2+ more active in presence of Mn2+ than Mg2+ Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration, minor poly(A) polymerase Bacillus subtilis
86000
-
 gel filtration, major poly(A) polymerase Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 two isoforms
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA primer required Bacillus subtilis diphosphate + RNA(A)n
-
 ?
ATP + RNA enzyme also has cleavage activity Bacillus subtilis diphosphate + RNA(A)n
-
 ?
ATP + RNA primer: poly(A) Bacillus subtilis diphosphate + RNA(A)n
-
 ?
ATP + RNA primer: dephosphorylated poly(A), tRNA Bacillus subtilis diphosphate + RNA(A)n
-
 ?