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Literature summary for 2.7.7.19 extracted from

  • Edmonds, M.
    Poly(A) adding enzymes (1982), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 15, 217-244.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2'-dATP
-
Bos taurus
2'-dATP
-
Coturnix sp.
2'-dATP
-
Cricetulus griseus
2'-dATP
-
Escherichia coli
2'-dATP
-
Homo sapiens
2'-dATP
-
Mus musculus
2'-dATP
-
Rattus norvegicus
2'-dATP
-
Vaccinia virus
3'-dATP
-
Bos taurus
3'-dATP
-
Coturnix sp.
3'-dATP
-
Cricetulus griseus
3'-dATP
-
Escherichia coli
3'-dATP
-
Homo sapiens
3'-dATP
-
Mus musculus
3'-dATP
-
Rattus norvegicus
3'-dATP
-
Vaccinia virus
CTP
-
Bos taurus
CTP
-
Coturnix sp.
CTP
-
Cricetulus griseus
CTP
-
Escherichia coli
CTP
-
Homo sapiens
CTP
-
Mus musculus
CTP
-
Rattus norvegicus
CTP
-
Vaccinia virus
diphosphate noncompetitive to ATP and primer Bos taurus
diphosphate noncompetitive to ATP and primer Coturnix sp.
diphosphate noncompetitive to ATP and primer Cricetulus griseus
diphosphate noncompetitive to ATP and primer Escherichia coli
diphosphate noncompetitive to ATP and primer Homo sapiens
diphosphate noncompetitive to ATP and primer Mus musculus
diphosphate noncompetitive to ATP and primer Rattus norvegicus
diphosphate noncompetitive to ATP and primer Vaccinia virus
GTP
-
Bos taurus
GTP
-
Coturnix sp.
GTP
-
Cricetulus griseus
GTP
-
Escherichia coli
GTP
-
Homo sapiens
GTP
-
Mus musculus
GTP
-
Rattus norvegicus
GTP
-
Vaccinia virus
K+ above 50 mM; KCl Bos taurus
K+ above 50 mM; KCl Coturnix sp.
K+ above 50 mM; KCl Cricetulus griseus
K+ above 50 mM; KCl Escherichia coli
K+ above 50 mM; KCl Homo sapiens
K+ above 50 mM; KCl Mus musculus
K+ above 50 mM; KCl Rattus norvegicus
K+ above 50 mM; KCl Vaccinia virus
additional information overview Bos taurus
additional information overview Coturnix sp.
additional information overview Cricetulus griseus
additional information overview Escherichia coli
additional information overview Homo sapiens
additional information overview Mus musculus
additional information overview Rattus norvegicus
additional information overview Vaccinia virus
N-ethylmaleimide inhibits Mn2+-activated enzyme Bos taurus
N-ethylmaleimide
-
Cricetulus griseus
N-ethylmaleimide inhibits Mn2+-activated enzyme Rattus norvegicus
Na+ above 50 mM; NaCl Bos taurus
Na+ above 50 mM; NaCl Coturnix sp.
Na+ above 50 mM; NaCl Cricetulus griseus
Na+ above 50 mM; NaCl Escherichia coli
Na+ above 50 mM; NaCl Homo sapiens
Na+ above 50 mM; NaCl Mus musculus
Na+ above 50 mM; NaCl Rattus norvegicus
Na+ above 50 mM; NaCl Vaccinia virus
phosphate
-
Bos taurus
phosphate
-
Escherichia coli
phosphate not: rat liver nuclear enzyme Rattus norvegicus
polyamines
-
Bos taurus
polyamines
-
Coturnix sp.
polyamines
-
Cricetulus griseus
polyamines
-
Escherichia coli
polyamines
-
Homo sapiens
polyamines
-
Mus musculus
polyamines
-
Rattus norvegicus
polyamines
-
Vaccinia virus
Proflavine only at very high levels Bos taurus
Proflavine only at very high levels Coturnix sp.
Proflavine only at very high levels Cricetulus griseus
Proflavine only at very high levels Escherichia coli
Proflavine only at very high levels Homo sapiens
Proflavine only at very high levels Mus musculus
Proflavine only at very high levels Rattus norvegicus
Proflavine only at very high levels Vaccinia virus
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Bos taurus
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Coturnix sp.
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Cricetulus griseus
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Escherichia coli
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Homo sapiens
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Mus musculus
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Rattus norvegicus
rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Vaccinia virus
spermine inhibition if poly(A), nuclear RNA, or tRNA serves as primer, not with short oligonucleotide primers such as (Ap)3A Rattus norvegicus
UTP
-
Bos taurus
UTP
-
Coturnix sp.
UTP
-
Cricetulus griseus
UTP
-
Escherichia coli
UTP
-
Homo sapiens
UTP
-
Mus musculus
UTP
-
Rattus norvegicus
UTP
-
Vaccinia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Cricetulus griseus
additional information
-
additional information
-
Mus musculus
additional information
-
additional information
-
Escherichia coli
additional information
-
additional information
-
Homo sapiens
additional information
-
additional information
-
Rattus norvegicus
additional information
-
additional information
-
Bos taurus
additional information
-
additional information
-
Vaccinia virus
additional information
-
additional information
-
Coturnix sp.
0.002
-
RNA primer
-
Escherichia coli
0.05
-
ATP
-
Cricetulus griseus
0.05
-
ATP
-
Escherichia coli
0.05
-
ATP p(A)3 primer, Mn2+-activated calf thymus enzyme Bos taurus
0.07
-
ATP
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bos taurus 5737
-
cytoplasm infected cytoplasm of HeLa cells Homo sapiens 5737
-
nucleus
-
Cricetulus griseus 5634
-
nucleus
-
Mus musculus 5634
-
nucleus
-
Escherichia coli 5634
-
nucleus
-
Homo sapiens 5634
-
nucleus
-
Rattus norvegicus 5634
-
nucleus
-
Bos taurus 5634
-
nucleus
-
Coturnix sp. 5634
-
viral nucleocapsid
-
Vaccinia virus 19013
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Cricetulus griseus
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Mus musculus
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Escherichia coli
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Homo sapiens
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Rattus norvegicus
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Bos taurus
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Vaccinia virus
Mg2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Coturnix sp.
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Cricetulus griseus
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Mus musculus
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Escherichia coli
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Homo sapiens
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Rattus norvegicus
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Bos taurus
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Vaccinia virus
Mn2+ divalent cation requirement may be fulfilled by Mn2+, Mg2+ or a combination of the two depending on the source of the enzyme Coturnix sp.
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Cricetulus griseus
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Mus musculus
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Escherichia coli
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Homo sapiens
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Rattus norvegicus
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Bos taurus
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Vaccinia virus
additional information overview: ion requirements, poly(A) polymerases purified from different sources, and in some cases even from the same source, respond differently to the presence of Mg2+ and Mn2+ Coturnix sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000 60000 sedimentation analysis Cricetulus griseus
48000
-
x * 48000, rat liver nucleoplasm, denaturing gel electrophoresis Rattus norvegicus
50000
-
x * 50000, Mg2+-activated, denaturing gel electrophoresis Homo sapiens
50000
-
x * 50000, denaturing gel electrophoresis Escherichia coli
58000
-
-
Escherichia coli
58000
-
Mg2+-activated enzyme, sedimentation analysis Homo sapiens
60000
-
-
Rattus norvegicus
60000
-
Mn2+-activated enzyme, sedimentation analysis, gel filtration Bos taurus
63000
-
Mn2+-activated, sedimentation analysis Homo sapiens
65000
-
embryo, sedimentation analysis, gel filtration Mus musculus
70000
-
enzyme from infected cytoplasm, glycerol density gradient sedimentation Vaccinia virus
75000
-
x * 75000, Mn2+-activated, denaturing gel electrophoresis Homo sapiens
80000
-
sucrose density gradient sedimentation Mus musculus
80000
-
sucrose density gradient sedimentation Vaccinia virus
120000 140000 Mg2+-activated enzyme, gel filtration Bos taurus
145000 155000 gel filtration Cricetulus griseus
150000
-
above, mouse L-cells, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RNA Cricetulus griseus overview of biological function ?
-
?
ATP + RNA Mus musculus overview of biological function ?
-
?
ATP + RNA Escherichia coli overview of biological function ?
-
?
ATP + RNA Homo sapiens overview of biological function ?
-
?
ATP + RNA Rattus norvegicus overview of biological function ?
-
?
ATP + RNA Bos taurus overview of biological function ?
-
?
ATP + RNA Vaccinia virus overview of biological function ?
-
?
ATP + RNA Coturnix sp. overview of biological function ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Bos taurus
-
2 forms of enzyme: Mn2+-activated and Mg2+-activated
-
Coturnix sp.
-
Quail
-
Cricetulus griseus
-
CHO fibroblasts
-
Escherichia coli
-
-
-
Homo sapiens
-
HeLa infected with vaccinia virus
-
Homo sapiens
-
2 forms of enzyme: Mn2+-activated, Mg2+-activated
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overview: purification methods Cricetulus griseus
overview: purification methods Mus musculus
overview: purification methods Escherichia coli
overview: purification methods Homo sapiens
overview: purification methods Rattus norvegicus
overview: purification methods Bos taurus
overview: purification methods Vaccinia virus
overview: purification methods Coturnix sp.

Reaction

Reaction Comment Organism Reaction ID
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Cricetulus griseus
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Mus musculus
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Escherichia coli
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Homo sapiens
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Rattus norvegicus
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Bos taurus
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Vaccinia virus
ATP + RNAn = diphosphate + RNAn+3'-A mechanism Coturnix sp.

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
Cricetulus griseus
-
embryo
-
Mus musculus
-
HeLa cell
-
Homo sapiens
-
hepatoma cell
-
Rattus norvegicus
-
L-cell
-
Mus musculus
-
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-
oviduct
-
Coturnix sp.
-
thymus
-
Homo sapiens
-
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA highly specific for ATP Cricetulus griseus diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Mus musculus diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Rattus norvegicus diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Vaccinia virus diphosphate + RNA(A)n
-
?
ATP + RNA highly specific for ATP Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA Mg2+-activated enzyme from calf thymus or HeLa cells prefers either longer poly(A) or RNAs rather than shorter oligomers of AMP Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA Mg2+-activated enzyme from calf thymus or HeLa cells prefers either longer poly(A) or RNAs rather than shorter oligomers of AMP Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Cricetulus griseus diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Mus musculus diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Rattus norvegicus diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Vaccinia virus diphosphate + RNA(A)n
-
?
ATP + RNA Mn2+-activated enzymes are indifferent to primer length Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA primer required Cricetulus griseus diphosphate + RNA(A)n
-
?
ATP + RNA primer required Mus musculus diphosphate + RNA(A)n
-
?
ATP + RNA primer required Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA primer required Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA primer required Rattus norvegicus diphosphate + RNA(A)n
-
?
ATP + RNA primer required Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA primer required Vaccinia virus diphosphate + RNA(A)n
-
?
ATP + RNA primer required Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Cricetulus griseus diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Mus musculus diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Rattus norvegicus diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Vaccinia virus diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA no specificity for the 3'-terminal nucleotides when poly(C) and poly(I), but not poly(U), primes poly(A) synthesis with the Mg2+-activated enzyme Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Cricetulus griseus diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Mus musculus diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Rattus norvegicus diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Vaccinia virus diphosphate + RNA(A)n
-
?
ATP + RNA other nucleotides polymerized at less than 1% of the ATP rate Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA adenosine 5'-(beta,gamma-methylene)triphosphate is efficiently polymerized into poly(A) with a polymerase from quail oviduct Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA Mg2+-activated calf thymus enzyme uses poly(A), tRNA, small RNA fragments from calf thymus RNA well, but HeLa 18 and 28S rRNA and MS-1 RNA poorly if at all Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA Mg2+-activated calf thymus enzyme uses poly(A), tRNA, small RNA fragments from calf thymus RNA well, but HeLa 18 and 28S rRNA and MS-1 RNA poorly if at all Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Cricetulus griseus diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Mus musculus diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Homo sapiens diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Rattus norvegicus diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Bos taurus diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Vaccinia virus diphosphate + RNA(A)n
-
?
ATP + RNA rather low specificity for primer Coturnix sp. diphosphate + RNA(A)n
-
?
ATP + RNA overview of biological function Cricetulus griseus ?
-
?
ATP + RNA overview of biological function Mus musculus ?
-
?
ATP + RNA overview of biological function Escherichia coli ?
-
?
ATP + RNA overview of biological function Homo sapiens ?
-
?
ATP + RNA overview of biological function Rattus norvegicus ?
-
?
ATP + RNA overview of biological function Bos taurus ?
-
?
ATP + RNA overview of biological function Vaccinia virus ?
-
?
ATP + RNA overview of biological function Coturnix sp. ?
-
?
ATP + RNA primer
-
Escherichia coli ?
-
?
additional information overview on substrates and primers Cricetulus griseus ?
-
?
additional information overview on substrates and primers Mus musculus ?
-
?
additional information overview on substrates and primers Escherichia coli ?
-
?
additional information overview on substrates and primers Homo sapiens ?
-
?
additional information overview on substrates and primers Rattus norvegicus ?
-
?
additional information overview on substrates and primers Bos taurus ?
-
?
additional information overview on substrates and primers Vaccinia virus ?
-
?
additional information overview on substrates and primers Coturnix sp. ?
-
?

Subunits

Subunits Comment Organism
?
-
Bos taurus
? x * 60000, hepatoma enzyme, SDS-PAGE Rattus norvegicus
? x * 48000, rat liver nucleoplasm, denaturing gel electrophoresis Rattus norvegicus
? x * 50000, Mg2+-activated, denaturing gel electrophoresis Homo sapiens
? x * 50000, denaturing gel electrophoresis Escherichia coli
? x * 75000, Mn2+-activated, denaturing gel electrophoresis Homo sapiens
monomer
-
Escherichia coli
monomer
-
Homo sapiens
monomer
-
Rattus norvegicus
monomer 1 * 60000, Mn2+-activated enzyme, denaturing gel electrophoresis Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 active in this range Cricetulus griseus
7 9 active in this range Mus musculus
7 9 active in this range Escherichia coli
7 9 active in this range Homo sapiens
7 9 active in this range Rattus norvegicus
7 9 active in this range Bos taurus
7 9 active in this range Vaccinia virus
7 9 active in this range Coturnix sp.