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2.7.4.3: adenylate kinase

This is an abbreviated version!
For detailed information about adenylate kinase, go to the full flat file.

Word Map on EC 2.7.4.3

Reaction

ATP
+
AMP
=
2 ADP

Synonyms

5'-AMP-kinase, ABC adenylate kinase, AD-004 like protein, adenosine 5'-triphosphate:adenosine 5'-monophosphate phosphotransferase, adenylate kinase, adenylate kinase 1, adenylate kinase 2, adenylate kinase 4, adenylate kinase 5, adenylate kinase 6, adenylate kinase 9, adenylate kinase isoenzyme 1, adenylate kinase isozyme 2, adenylate kinase-2, adenylate kinase-like protein 1, adenylic kinase, adenylokinase, ADK, ADK1, ADK2, ADLP, AK1, AK2, AK4, AK5, AK5p1, AK5p2, AK6, AK7, AK8, AK9, AKE, AKlse4, AKm, AKmeso, AKp, AKpsycrho, AKthermo, ATP:AMP phosphotransferase, CFTR, CINAP, coilin interacting nuclear ATPase protein, cystic fibrosis transmembrane conductance regulator, DAK2, Dak6, kinase, adenylate (phosphorylating), kinase, myo- (phosphorylating), MeADK1, MeADK2, MJ0458, myokinase, nonstructural protein 4B, NS4B, pfSMCnbd, phosphotransferase, Rv0733, Saci_0573, SpAdK, structural maintenance of chromosome protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.3 adenylate kinase

Storage Stability

Storage Stability on EC 2.7.4.3 - adenylate kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, partially purified, more than 1 month
-
-20°C, several weeks
-20°C, up to 4 months
-
-80°C, 22 mM Tris-HCl, 0.5 M NaCl, pH 7.8, a few months
-
0-4°C, 1 month
-
0°C, a few days stable, after which activity drops to 70%, this lower specific activity can be maintained at -20°C in ammonium sulfate solution for a prolonged period
-
0°C, at least 2 days
-
0°C, at least 7 days
3°C, 5-10% loss within 1 month
-
4°C, 0.5 mg enzyme/ml, in 100 mM Tris-HCl buffer, pH 7.4, 7 days
-
4°C, 30% or 70% loss of activity at pH 6 or pH 5, respectively, on standing overnight
-
4°C, 5 mM sodium phosphate, pH 7, 1 mM 2-mercaptoethanol, 1 month
-
4°C, in distilled water, 6 weeks
-
4°C, pH 6-9, 2 weeks
-
deep frozen, 10-13% loss of activity within 6 months
-
deep frozen, pH 6-9, 20 mg enzyme/ml, 4 months, but 60% loss of activity within a few days at pH 5 and 10
-
deep frozen, several weeks
frozen enzyme solutions or lyophilized powders, wild-type and mutant enzymes, stable for weeks
-
frozen, in 100 mM Tris-HCl buffer, pH 7, 60% glycerol, several months
-
frozen, up to 6 months
-
room temperature, at least 2 days
-