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2.7.4.3: adenylate kinase

This is an abbreviated version!
For detailed information about adenylate kinase, go to the full flat file.

Word Map on EC 2.7.4.3

Reaction

ATP
+
AMP
=
2 ADP

Synonyms

5'-AMP-kinase, ABC adenylate kinase, AD-004 like protein, adenosine 5'-triphosphate:adenosine 5'-monophosphate phosphotransferase, adenylate kinase, adenylate kinase 1, adenylate kinase 2, adenylate kinase 4, adenylate kinase 5, adenylate kinase 6, adenylate kinase 9, adenylate kinase isoenzyme 1, adenylate kinase isozyme 2, adenylate kinase-2, adenylate kinase-like protein 1, adenylic kinase, adenylokinase, ADK, ADK1, ADK2, ADLP, AK1, AK2, AK4, AK5, AK5p1, AK5p2, AK6, AK7, AK8, AK9, AKE, AKlse4, AKm, AKmeso, AKp, AKpsycrho, AKthermo, ATP:AMP phosphotransferase, CFTR, CINAP, coilin interacting nuclear ATPase protein, cystic fibrosis transmembrane conductance regulator, DAK2, Dak6, kinase, adenylate (phosphorylating), kinase, myo- (phosphorylating), MeADK1, MeADK2, MJ0458, myokinase, nonstructural protein 4B, NS4B, pfSMCnbd, phosphotransferase, Rv0733, Saci_0573, SpAdK, structural maintenance of chromosome protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.3 adenylate kinase

Temperature Stability

Temperature Stability on EC 2.7.4.3 - adenylate kinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-12
Megalodesulfovibrio gigas
-
maximum stability
103
Tm-value, wild-type enzyme
25
-
20 h stable, pH 6-9
30
-
above, 10 min, rapid loss of activity
30.7
T-value, mutant enzyme T188K
33.8
T-value, wild-type enzyme
36
T-value, mutant enzyme S48A
36.6
T-value, mutant enzyme V118I
38.3
Tm-value, mutant enzyme T26N
38.4
Tm-value, mutant enzyme T26A
38.7
Tm-value, mutant enzyme T26S
38.8
T-value, mutant enzyme V28I
39
-
t1/2: 11 min
39.4
Tm-value, mutant enzyme I26T
40
1 h, the enzyme maintains 77.5% residual activity compared with the enzyme stored on ice
40.8
-
T-value, wild-type enzyme
41.4
T-value, mutant enzyme V118I/V173I
42.7
Tm-value, wild-type enzyme
43.7 - 45.3
Megalodesulfovibrio gigas
the melting temperatures of Zn2+-, Co2+-, and Fe2+-containing recombinant adenylate kinases are at 45.3, 43.7, and 45.0°C, respectively
44.1
Tm-value, mutant enzyme T26Y
46
30 min, 50% inactivation
46.4
Tm-value, wild-type enzyme
48.3
Tm-value, mutant enzyme T26V
48.4
Tm-value, mutant enzyme T26L
50
-
enzyme type 2: in 10 mM sodium citrate buffer, pH 6, t1/2: 5 min, enzyme type 1: t1/2: 31 min
50.8
Tm-value, mutant enzyme T26I
52
-
the melting temperature of the free enzyme is at 52°C
54
XP_019937160.1
30 min, 50% inactivation
55
-
24 h, stable at pH 6
55.8
T-value, wild-type enzyme, in presence of P1,P5-di(adenosine 5')-pentaphosphate
56.5
T-value, mutant enzyme I28V/I118V/I173V, in presence of P1,P5-di(adenosine 5')-pentaphosphate
59
1 h, the enzyme maintains 47% residual activity compared with the enzyme stored on ice
60.3
T-value, mutant enzyme V28I/V118I/V173I, in presence of P1,P5-di(adenosine 5')-pentaphosphate
61.8
T-value, wild-type enzyme, in presence of P1,P5-di(adenosine 5')-pentaphosphate
65 - 70
-
12 h, 16% loss of activity, 24 h, 23% loss of activity, variation of ionic strength or addition of substrates does not stabilize
69
Tm-value, wild-type enzyme
73
Tm-value, mutant enzyme J36V
83
inactivation of the enzyme in 100 mM KCl is too rapid at 83°C for an accurate assessment of the effects of pressure. In the presence of 800 mM KCl, it is clear that the imposition of 50-MPa pressure has a destabilizing influence
86
-
Tm-value, wild-type enzyme
93.5
melting point EDTA-treated
96
Tm-value, mutant enzyme V160J
98
Tm-value, mutant enzyme J36V
99
-
the melting temperature of the free enzyme is at 99°C
99.1
melting temperature
99.7
melting point holoenzyme
additional information