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2.7.4.3: adenylate kinase

This is an abbreviated version!
For detailed information about adenylate kinase, go to the full flat file.

Word Map on EC 2.7.4.3

Reaction

ATP
+
AMP
=
2 ADP

Synonyms

5'-AMP-kinase, ABC adenylate kinase, AD-004 like protein, adenosine 5'-triphosphate:adenosine 5'-monophosphate phosphotransferase, adenylate kinase, adenylate kinase 1, adenylate kinase 2, adenylate kinase 4, adenylate kinase 5, adenylate kinase 6, adenylate kinase 9, adenylate kinase isoenzyme 1, adenylate kinase isozyme 2, adenylate kinase-2, adenylate kinase-like protein 1, adenylic kinase, adenylokinase, ADK, ADK1, ADK2, ADLP, AK1, AK2, AK4, AK5, AK5p1, AK5p2, AK6, AK7, AK8, AK9, AKE, AKlse4, AKm, AKmeso, AKp, AKpsycrho, AKthermo, ATP:AMP phosphotransferase, CFTR, CINAP, coilin interacting nuclear ATPase protein, cystic fibrosis transmembrane conductance regulator, DAK2, Dak6, kinase, adenylate (phosphorylating), kinase, myo- (phosphorylating), MeADK1, MeADK2, MJ0458, myokinase, nonstructural protein 4B, NS4B, pfSMCnbd, phosphotransferase, Rv0733, Saci_0573, SpAdK, structural maintenance of chromosome protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.3 adenylate kinase

Purification

Purification on EC 2.7.4.3 - adenylate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 allelozymes: AK1*1 and AK1*2
-
2 isozymes, partial
-
acidic isozyme
-
Affi-Gel Blue Gel column chromatography, Q-Sepharose column chromatography, and S-200 gel filtration
-
ammonium sulfate fractionation and Q-Sepharose column chromatography
ammonium sulfate precipitation, Blue Sepharose column chromatography, and Superdex 75 gel filtration
Megalodesulfovibrio gigas
and their multiple forms
-
Blue Sepharose column chromatography and Superdex 75 gel filtration
Megalodesulfovibrio gigas
column chromatographies on TOYOPEARL SP-650 M and Superdex 75 10/300
glutathione-Sepharose column chromatography
GSTrap 4B column chromatography and Superdex 75 gel filtration
His Bind column chromatography
HiTrap Q-XL Sepharose column chromatography, Affi-Gel blue resin colum chromatography, and Superdex-200 gel filtration
liver enzyme, 4 isozymes
-
liver mitochondria, eye lens
-
mitochondrial enzyme
-
muscle
muscle and liver enzyme
-
Ni-affinity column chromatography and Superdex-75 gel filtration
-
Ni-NTA bead chromatography, and glutathione Sepharose bead chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
nickel affinity column chromatography and Superdex 200 gel filtration
-
no isozymes
-
nucleotide-binding domain 1 and 2, purified to homogeneity under denaturing conditions
nucleotide-binding domain 1 of CFTR purified to homogeneity by nickel ion affinity chromatography, followed by Sepharose S200 gel filtration and a second nickel affinity step
overexpressed in E. coli
-
partial
partial, to near homogeneity
predominant form AKalpha (major form of AK-1 isozymes)
-
Protino-Ni-TED column chromatography
Q14EL6
Q-XL Sepharose column chromatography, Affi-Gel Blue resin column chromatography, and Superdex-200 gel filtration
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration, the GST moiety is cleaved using tobacco etch virus protease
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The tag is cleaved using tobacco etch virus protease, followed by dialysis, gel filtration, and ultrafiltration
recombinant His-tagged mutant AKm1 and AKm2 enzymes from Escherichia coli by nickel affinity chromatography and gel filtration
separable by electrophoresis, not by isoelectric focusing
-
Sepharose column chromatography and Superdex 75 gel filtration
single-step purification procedure from overproducing strain GT836
-
TALON metal affinity resin column chromatography