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2.7.4.22: UMP kinase

This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.

Reaction

ATP
+
UMP
=
ADP
 +
UDP

Synonyms

ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.22 UMP kinase

Temperature Stability

Temperature Stability on EC 2.7.4.22 - UMP kinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
half-maximal inactivation
43
mutant T138A/N140A, temperature of half-inactivation
48
Tm for mutant D201N
51
mutant D201N is half-inactivated
52
Tm for mutant D77N
55
Tm of mutant R11H
56
mutant T138A, temperature of half-inactivation
57
Tm of mutant G232D
59
mutant N140A, temperature of half-inactivation
59 - 62
mutants R62H, D146N, D168N and D174N are half inactivated between 59 and 62°C
60 - 75
the enzyme shows Tm values of 60°C in the absence of nucleotides and 65°C and 75°C with 1 mM GTP and UTP, respectively
62.7
Tm in the presence of 0.1 mM GTP
63.7
Tm in the presence of 1 mM ATP
64
wild-type protein is half-inactivated
65.8
Tm in the presence of 1 mM GTP
68
wild-type, temperature of half-inactivation
69.8
Tm in the presence of 5 mM GTP
70
half-maximal inactivation in the presence of 1 mM UTP
73
Tm for mutant D159N
74.6
Tm in the presence of 0.1 mM UTP
75.6
Tm in the presence of 1 mM UMP
82.4
Tm in the presence of 1 mM UTP
86.5
Tm in the presence of 5 mM UTP
additional information