Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.4.22: UMP kinase

This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.

Reaction

ATP
+
UMP
=
ADP
 +
UDP

Synonyms

ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.22 UMP kinase

Storage Stability

Storage Stability on EC 2.7.4.22 - UMP kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 12 months storage, less than 10% loss of activity
0°C, 6 h, less than 10% loss of activity
4°C, 50 mM Tris-HCl (pH 7.4) as insoluble proteins, after solubilization with 0.1 M borate (pH 9) or with 1 mM UTP in 50 mM Tris-HCl (pH 7.4), the enzyme is fully active
4°C, 50 mM Tris-HCl (pH 7.4), several months, no significant loss of activity. An exception is mutant D174N, which loses two-thirds of its activity after 3 months at 4°C
4°C, in 20 mM phosphate (pH 7.0) and 100 mM NaCl, about 2 months, no apparent loss of activity
room temperature, 0.1 M borate buffer (pH 8.5), 2 months, no loss of activity
room temperature, 50 mM Tris-HCl (pH 7.4), 0.1 M NaCl, 2 mM UTP, 2 weeks, no loss of activity