2.7.4.22: UMP kinase
This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.
Reaction
Synonyms
ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936
ECTree
2.7.4.22 UMP kinaseAdvanced search results
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results (Activating Compound
Activating Compound on EC 2.7.4.22 - UMP kinase
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ACTIVATING COMPOUND 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
5-fluoro-UTP
below 0.15 mM 5-fluoro-UTP increases the activity of the enzyme by 80%
dialdehyde-GTP
slight stimulatory effect, 30% maximal increase in UMP-kinase activity
GMP-4-nitrophenol
much weaker activator (only 50% increase in activity at 1 mM)
GMP
much weaker activator (only 30% increase in activity at 2.5 mM)
GTP
Q81S73
UTP inhibition, but not GTP activation, is sensitive to Mg2+ levels
GTP
half-maximum activation is reached at 0.1 mM GTP, in the absence of GTP the enzyme exhibits less than 10% of its maximum activity
GTP
0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP
GTP
allosteric activator, the GTP-binding site, situated at 15 A from the UMP-binding site and at 24 A from the ATP-binding site, is delineated by two contiguous dimers. movements induced by binding GTP, overview. UTP and GTP are tightly coupled in both wild-type enzyme and N140A variant
GTP
GTP increases the reaction rate by a factor of 3.5, with half-saturation at 0.1 mM
GTP
0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP
additional information
GMP-PCP and 8-bromo-GTP are ineffective as activators
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additional information
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GMP-PCP and 8-bromo-GTP are ineffective as activators
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additional information
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GTP no influence, GTP does not bind, unlike most bacteria, is not activated by GTP, UTP acts as a competitive inhibitor against both substrates, high concentration of UMP abolishes the inhibition of UTP at low ATP concentrations, indicating that UTP binds to the acceptor site (UMP site)
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additional information
GTP no influence, GTP does not bind, unlike most bacteria, is not activated by GTP, UTP acts as a competitive inhibitor against both substrates, high concentration of UMP abolishes the inhibition of UTP at low ATP concentrations, indicating that UTP binds to the acceptor site (UMP site)
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additional information
GTP has no detectable effect on UpUMPK activity unlike UMPKs from other bacteria
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additional information
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GTP has no detectable effect on UpUMPK activity unlike UMPKs from other bacteria
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additional information
no activation of wild-type enzyme and F133 mutants by GTP
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additional information
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no activation of wild-type enzyme and F133 mutants by GTP
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