Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.99.19: undecaprenyl-diphosphooligosaccharide-protein glycotransferase

This is an abbreviated version!
For detailed information about undecaprenyl-diphosphooligosaccharide-protein glycotransferase, go to the full flat file.

Word Map on EC 2.4.99.19

Reaction

tritrans,heptacis-undecaprenyl diphosphooligosaccharide
+
[protein]-L-asparagine
=
tritrans,heptacis-undecaprenyl diphosphate
 +
a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine

Synonyms

bacterial oligosaccharyltransferase, bacterial OST, N-oligosaccharyltransferase, N-OTase, oligosaccharyl transferase, oligosaccharyltransferase, oligosacharyltransferase, OST, OT, OTase, PglB, PglB oligosaccharyltransferase, PglB protein, PglB1, PglB2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.99 Transferring other glycosyl groups
                2.4.99.19 undecaprenyl-diphosphooligosaccharide-protein glycotransferase

Cloned

Cloned on EC 2.4.99.19 - undecaprenyl-diphosphooligosaccharide-protein glycotransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-Gold (DE3) cells
-
gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli
gene pglB, expression of His10-tagged wild-type and mutant enzymes in Escherichia coli strain SCM6
gene pglB, expression of the GST-tagged C-terminal globular domain of PglB in Escherichia coli strain BL21(DE3)pLysS, expression of His-tagged full-length PglB in Escherichia coli strain BL21(DE3)
gene pglB, expression of the wild-type and mutant WAAYGY-PglB variant in Escherichia coli in membranes
-
gene pglB, functional expression in Escherichia coli
-
gene pglB, functional expression in Escherichia coli strains E69, CWG28, or CWG44 using an arabinose-inducible promoter, coexpression of the Acra protein substrate
-
gene pglB, large-scale overexpression of His-tagged PglB in Escherichia coli strain C43(DE3)
-
gene pglB, sequence and structure comparison with STT3 subunit of the eukaryotic OTase complex of Saccharomyces cerevisiae
-
gene pglB, sequence comparisons and phylogenetic analysis, cloning and analysis of an operon that might encode all enzymes required for N-glycosylation in Desulfovibrio desulfuricans, functional expression of gene pglB in Eschericia coli
-
gene pglB, sequence comparisons and phylogenetic analysis, functional expression in Escherichia coli. All genes necessary for N-linked glycosylation in Campylobacter jejuni are located in a single locus
-
genes pglB1 and pglB2, DNA and amino acid sequence determination and analysis. Gene pglB1, but not the pglB2, gene is able to partially complement the Campylobacter jejuni pglB gene
-