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Literature summary for 2.4.99.19 extracted from

  • Ielmini, M.V.; Feldman, M.F.
    Desulfovibrio desulfuricans PglB homolog possesses oligosaccharyltransferase activity with relaxed glycan specificity and distinct protein acceptor sequence requirements (2011), Glycobiology, 21, 734-742.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pglB, sequence comparisons and phylogenetic analysis, cloning and analysis of an operon that might encode all enzymes required for N-glycosylation in Desulfovibrio desulfuricans, functional expression of gene pglB in Eschericia coli Desulfovibrio desulfuricans
gene pglB, sequence comparisons and phylogenetic analysis, functional expression in Escherichia coli. All genes necessary for N-linked glycosylation in Campylobacter jejuni are located in a single locus Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Desulfovibrio desulfuricans
-
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Campylobacter jejuni
-
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
gene pglB
-
Desulfovibrio desulfuricans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PglBCj adds two glycan chains to model protein acceptor AcrA. PglBCj recognizes the conventional bacterial glycosylation sequon consisting of the sequence D/E-X1-NNX2-S/T, where X1 and X2 are any amino acid except proline, in contrast to PglBDd from Desulfovibrio desulfuricans, overview. AcrA contains five potential N-glycosylation sites N-X-S/T, but only the two that have an Asp residue at the -2 position are recognized by the Campylobacter jejuni enzyme, N123 and N273 Campylobacter jejuni ?
-
?
additional information the recombinantly expressed PglB can transfer the Campylobacter jejuni heptasaccharide onto the model protein acceptor AcrA, PglBDd adds a single glycan chain to AcrA. PglBDd does not recognize the conventional bacterial glycosylation sequon consisting of the sequence D/E-X1-NNX2-S/T, where X1 and X2 are any amino acid except proline, and instead uses a different site for the attachment of the oligosaccharide than PglBCj from Campylobacter jejuni, PglBDd exhibits relaxed glycan specificity, being able to transfer mono- and polysaccharides to AcrA, overview Desulfovibrio desulfuricans ?
-
?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [HmcA]-L-asparagine a protein from Desulfovibrio gigas, N-glycosylation site is N261 Desulfovibrio desulfuricans tritrans,heptacis-undecaprenyl diphosphate + [HmcA]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
Desulfovibrio desulfuricans tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine PglBDd can transfer mono- and polysaccharides. LLO and AcrA and substrate glycosylation sites, overview. PglBDd does not require a negatively charged residue in the -2 position of the glycosylation sequon N-X-S/T Desulfovibrio desulfuricans tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Subunits

Subunits Comment Organism
More STT3 catalytic subunit sequence comparisons, overview Desulfovibrio desulfuricans
More STT3 catalytic subunit sequence comparisons, overview Campylobacter jejuni

Synonyms

Synonyms Comment Organism
N-OTase
-
Desulfovibrio desulfuricans
N-OTase
-
Campylobacter jejuni
oligosaccharyltransferase
-
Desulfovibrio desulfuricans
oligosaccharyltransferase
-
Campylobacter jejuni
OTase
-
Desulfovibrio desulfuricans
OTase
-
Campylobacter jejuni
PglB
-
Desulfovibrio desulfuricans
PglB
-
Campylobacter jejuni

General Information

General Information Comment Organism
evolution in the delta proteobacteria Desulfovibrio sp., the PglB homologue is more closely related to eukaryotic and archaeal OTases than to its Campylobacter counterparts Desulfovibrio desulfuricans