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Literature summary for 2.4.99.19 extracted from

  • Maita, N.; Nyirenda, J.; Igura, M.; Kamishikiryo, J.; Kohda, D.
    Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases (2010), J. Biol. Chem., 285, 4941-4950.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pglB, expression of the GST-tagged C-terminal globular domain of PglB in Escherichia coli strain BL21(DE3)pLysS, expression of His-tagged full-length PglB in Escherichia coli strain BL21(DE3) Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
purified detagged and methylated wild-type and selenomethionine-labeled C-terminal globular domain of PglB, hanging drop vapour diffusion method, 500 nl of 10 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed in an 1:1 ratio with reservoir solution containing 0.1 M sodium cacodylate, pH 6.5, 18% PEG 8000, 0.2 M calcium acetate, 20°C, X-ray diffraction structure determination and analysis at 2.8 A resolution Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Campylobacter jejuni 16020
-

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q5HTX9 gene pglB
-
Campylobacter jejuni RM1221 Q5HTX9 gene pglB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length PglB from Escherichia coli strain BL21(DE3) in the membrane fraction by ultracentrifugation, recombinant GST-tagged wild-type and selenomethionine-labeled C-terminal globular domain of PglB from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography and cleavage of the tag by 3C protease, followed by to reductive methylation of the lysine residues, gel filtration, and anion exchange chromatography Campylobacter jejuni

Subunits

Subunits Comment Organism
monomer
-
Campylobacter jejuni
More C-terminal globular domain of PglB compared with the structure from the archaeon Pyrococcus AglB, overview Campylobacter jejuni

Synonyms

Synonyms Comment Organism
oligosaccharyltransferase
-
Campylobacter jejuni
OST
-
Campylobacter jejuni
PglB
-
Campylobacter jejuni

General Information

General Information Comment Organism
evolution bacterial PglB and archaeal AglB constitute a protein family of the catalytic subunit of OST, along with STT3 from eukaryotes, that has three types of OST catalytic centers, structure analysis and comparison, overview Campylobacter jejuni
additional information the PglB structure reveals a distinct catalytic motif in the transmembrane region that contributes to the catalytic function, structure analysis, overview Campylobacter jejuni