Cloned (Comment) | Organism |
---|---|
gene pglB, large-scale overexpression of His-tagged PglB in Escherichia coli strain C43(DE3) | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | topological model of PglB with periplasmic, transmembrane, and cytoplasmic parts, overview | Campylobacter jejuni | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Campylobacter jejuni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
x * 82000, SDS-PAGE | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene pglB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PglB from Escherichia coli strain C43(DE3) by nickel affinity chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Asp-Phe-Asn-Val-Ser-Lys-Ala + N-acetyl-alpha-D-galactosaminyl-diphospho-tritrans,heptacis-undecaprenol | a chemically synthesized sugar donor with synthesized peptide acceptor | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + Lys-Asp-Phe-N4-[N-acetyl-beta-D-galactosaminyl]-Asn-Val-Ser-Lys-Ala | - |
? | |
additional information | PglB is solely responsible for the oligosaccharyltransferase activity, PglB exhibits relaxed sugar substrate specificity | Campylobacter jejuni | ? | - |
? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, SDS-PAGE | Campylobacter jejuni |
More | PglB is a protein with two domains, an N-terminal transmembrane domain with 9-13 predicted transmembrane segments, and a C-terminal soluble domain which contains the catalytic center | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
bacterial oligosaccharyltransferase | - |
Campylobacter jejuni |
PglB | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
additional information | topology mapping of PglB using the PhoA/LacZ fusion method, overview. The topological model shows that PglB possesses 11 transmembrane segments and two relatively large periplasmic regions other than the C-terminal domain, which is consistent with the proposal of the common Ncyt-Cperi topology with 11 transmembrane segments for the STT3 family proteins | Campylobacter jejuni |