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Literature summary for 2.4.99.19 extracted from

  • Li, L.; Woodward, R.; Ding, Y.; Liu, X.W.; Yi, W.; Bhatt, V.S.; Chen, M.; Zhang, L.W.; Wang, P.G.
    Overexpression and topology of bacterial oligosaccharyltransferase PglB (2010), Biochem. Biophys. Res. Commun., 394, 1069-1074.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pglB, large-scale overexpression of His-tagged PglB in Escherichia coli strain C43(DE3) Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane topological model of PglB with periplasmic, transmembrane, and cytoplasmic parts, overview Campylobacter jejuni 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 82000, SDS-PAGE Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Campylobacter jejuni
-
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
gene pglB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PglB from Escherichia coli strain C43(DE3) by nickel affinity chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Asp-Phe-Asn-Val-Ser-Lys-Ala + N-acetyl-alpha-D-galactosaminyl-diphospho-tritrans,heptacis-undecaprenol a chemically synthesized sugar donor with synthesized peptide acceptor Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + Lys-Asp-Phe-N4-[N-acetyl-beta-D-galactosaminyl]-Asn-Val-Ser-Lys-Ala
-
?
additional information PglB is solely responsible for the oligosaccharyltransferase activity, PglB exhibits relaxed sugar substrate specificity Campylobacter jejuni ?
-
?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Campylobacter jejuni
More PglB is a protein with two domains, an N-terminal transmembrane domain with 9-13 predicted transmembrane segments, and a C-terminal soluble domain which contains the catalytic center Campylobacter jejuni

Synonyms

Synonyms Comment Organism
bacterial oligosaccharyltransferase
-
Campylobacter jejuni
PglB
-
Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Campylobacter jejuni

General Information

General Information Comment Organism
additional information topology mapping of PglB using the PhoA/LacZ fusion method, overview. The topological model shows that PglB possesses 11 transmembrane segments and two relatively large periplasmic regions other than the C-terminal domain, which is consistent with the proposal of the common Ncyt-Cperi topology with 11 transmembrane segments for the STT3 family proteins Campylobacter jejuni