Cloned (Comment) | Organism |
---|---|
genes pglB1 and pglB2, DNA and amino acid sequence determination and analysis. Gene pglB1, but not the pglB2, gene is able to partially complement the Campylobacter jejuni pglB gene | Helicobacter pullorum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | insertional knockout mutagenesis of the gene pglB2 is unsuccessful | Helicobacter pullorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Campylobacter jejuni | |
Mn2+ | activates | Helicobacter pullorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Campylobacter jejuni | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | ? | - |
? | |
additional information | Helicobacter pullorum | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | ? | - |
? | |
additional information | Helicobacter pullorum NCTC 12824 | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | ? | - |
? | |
additional information | Campylobacter jejuni NCTC 11168 | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | ? | - |
? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Helicobacter pullorum | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Helicobacter pullorum NCTC 12824 | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni NCTC 11168 | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
single gene pglB | - |
Campylobacter jejuni NCTC 11168 | - |
single gene pglB | - |
Helicobacter pullorum | - |
the organism contains two unrelated pglB genes, pglB1 and pglB2, neither of which is located within a larger locus involved in protein glycosylation | - |
Helicobacter pullorum NCTC 12824 | - |
the organism contains two unrelated pglB genes, pglB1 and pglB2, neither of which is located within a larger locus involved in protein glycosylation | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | Campylobacter jejuni | ? | - |
? | |
additional information | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | Helicobacter pullorum | ? | - |
? | |
additional information | PglB-dependent N-glycosylation, structural analysis of in vitro-generated glycopeptides, overview | Campylobacter jejuni | ? | - |
? | |
additional information | PglB1-dependent N glycosylation with a linear pentasaccharide requiring an acidic residue at the -2 position of the N-glycosylation sequon, structural analysis of in vitro-generated glycopeptides, overview | Helicobacter pullorum | ? | - |
? | |
additional information | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | Helicobacter pullorum NCTC 12824 | ? | - |
? | |
additional information | PglB1-dependent N glycosylation with a linear pentasaccharide requiring an acidic residue at the -2 position of the N-glycosylation sequon, structural analysis of in vitro-generated glycopeptides, overview | Helicobacter pullorum NCTC 12824 | ? | - |
? | |
additional information | PglB1 protein, but not PglB2, is able to transfer Campylobacter jejuni N-linked glycan onto an acceptor protein in Escherichia coli | Campylobacter jejuni NCTC 11168 | ? | - |
? | |
additional information | PglB-dependent N-glycosylation, structural analysis of in vitro-generated glycopeptides, overview | Campylobacter jejuni NCTC 11168 | ? | - |
? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Helicobacter pullorum | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | e.g. Campylobacter jejuni glycoprotein (Cj0114) contaiing four potential N-glycosylation sites | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | e.g. Campylobacter jejuni glycoprotein (Cj0114) contaiing four potential N-glycosylation sites | Helicobacter pullorum | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Helicobacter pullorum NCTC 12824 | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | e.g. Campylobacter jejuni glycoprotein (Cj0114) contaiing four potential N-glycosylation sites | Helicobacter pullorum NCTC 12824 | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni NCTC 11168 | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | e.g. Campylobacter jejuni glycoprotein (Cj0114) contaiing four potential N-glycosylation sites | Campylobacter jejuni NCTC 11168 | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Synonyms | Comment | Organism |
---|---|---|
PglB | - |
Campylobacter jejuni |
PglB1 | - |
Helicobacter pullorum |
PglB2 | - |
Helicobacter pullorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Campylobacter jejuni |
30 | - |
assay at | Helicobacter pullorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Campylobacter jejuni |
7 | - |
assay at | Helicobacter pullorum |
General Information | Comment | Organism |
---|---|---|
malfunction | insertional knockout mutagenesis of the gene pglB2 is unsuccessful, suggesting that it is essential | Helicobacter pullorum |
physiological function | the key enzyme involved in the coupling of glycan to asparagine residues within the acceptor sequon of the glycoprotein is the oligosaccharyltransferase PglB | Campylobacter jejuni |
physiological function | the key enzyme involved in the coupling of glycan to asparagine residues within the acceptor sequon of the glycoprotein is the oligosaccharyltransferase PglB | Helicobacter pullorum |