Cloned (Comment) | Organism |
---|---|
gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli | Campylobacter jejuni |
gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli | Campylobacter lari |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Campylobacter jejuni | PglBCj can glycosylate residues in both unstructured and structured regions of eukaryotic acceptor proteins in vivo | ? | - |
? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter lari | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene pglB | - |
Campylobacter lari | - |
gene pglB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PglBCj can glycosylate residues in both unstructured and structured regions of eukaryotic acceptor proteins in vivo | Campylobacter jejuni | ? | - |
? | |
additional information | PglBCj is able to transfer Man3GlcNAc2 to extended sites but not to minimal glycosylation sites in engineered or eukaryotic target proteins | Campylobacter jejuni | ? | - |
? | |
additional information | PglBCl is able to transfer Man3GlcNAc2 to extended sites but not to minimal glycosylation sites in engineered or eukaryotic target proteins | Campylobacter lari | ? | - |
? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter lari | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | as glycoprotein targets are used Escherichia coli maltose binding protein which is a native periplasmic protein and the anti-beta-galactosidase single-chain antibody fragment called scFv13-R4 that is modified with an N-terminal cotranslational export signal from Escherichia coli DsbA. The proteins are each modified C-terminally with four tandem repeats of the bacterial glycan acceptor motif DQNAT and recombinantly expressed, overview | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | as glycoprotein targets are used Escherichia coli maltose binding protein which is a native periplasmic protein and the anti-beta-galactosidase single-chain antibody fragment called scFv13-R4 that is modified with an N-terminal cotranslational export signal from Escherichia coli DsbA. The proteins are each modified C-terminally with four tandem repeats of the bacterial glycan acceptor motif DQNAT and recombinantly expressed, overview | Campylobacter lari | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Synonyms | Comment | Organism |
---|---|---|
OTase | - |
Campylobacter jejuni |
OTase | - |
Campylobacter lari |
PglB | - |
Campylobacter jejuni |
PglB | - |
Campylobacter lari |