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Literature summary for 2.4.99.19 extracted from

  • Valderrama-Rincon, J.D.; Fisher, A.C.; Merritt, J.H.; Fan, Y.Y.; Reading, C.A.; Chhiba, K.; Heiss, C.; Azadi, P.; Aebi, M.; DeLisa, M.P.
    An engineered eukaryotic protein glycosylation pathway in Escherichia coli (2012), Nat. Chem. Biol., 8, 434-436.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli Campylobacter jejuni
gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli Campylobacter lari

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Campylobacter jejuni PglBCj can glycosylate residues in both unstructured and structured regions of eukaryotic acceptor proteins in vivo ?
-
?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Campylobacter jejuni
-
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Campylobacter lari
-
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
gene pglB
-
Campylobacter lari
-
gene pglB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PglBCj can glycosylate residues in both unstructured and structured regions of eukaryotic acceptor proteins in vivo Campylobacter jejuni ?
-
?
additional information PglBCj is able to transfer Man3GlcNAc2 to extended sites but not to minimal glycosylation sites in engineered or eukaryotic target proteins Campylobacter jejuni ?
-
?
additional information PglBCl is able to transfer Man3GlcNAc2 to extended sites but not to minimal glycosylation sites in engineered or eukaryotic target proteins Campylobacter lari ?
-
?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
Campylobacter lari tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine as glycoprotein targets are used Escherichia coli maltose binding protein which is a native periplasmic protein and the anti-beta-galactosidase single-chain antibody fragment called scFv13-R4 that is modified with an N-terminal cotranslational export signal from Escherichia coli DsbA. The proteins are each modified C-terminally with four tandem repeats of the bacterial glycan acceptor motif DQNAT and recombinantly expressed, overview Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine as glycoprotein targets are used Escherichia coli maltose binding protein which is a native periplasmic protein and the anti-beta-galactosidase single-chain antibody fragment called scFv13-R4 that is modified with an N-terminal cotranslational export signal from Escherichia coli DsbA. The proteins are each modified C-terminally with four tandem repeats of the bacterial glycan acceptor motif DQNAT and recombinantly expressed, overview Campylobacter lari tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Synonyms

Synonyms Comment Organism
OTase
-
Campylobacter jejuni
OTase
-
Campylobacter lari
PglB
-
Campylobacter jejuni
PglB
-
Campylobacter lari