2.4.1.101: alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.101

2.4.1.101

n-glycans

n-linked

glycosyltransferases

mannosidase

oligomannose

high-mannose

complex-type

paucimannose

swainsonine

golgi-resident

beta-1,4-galactosyltransferase

paucimannosidic

man3glcnac2

medial-golgi

glcnacman5glcnac2

biotechnology

medicine

molecular biology

Reaction

Synonyms

acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-, alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, alpha3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, AtGnTI, beta-1-2 GlcNAc transferase, ChGnTI, EC 2.4.1.51, GlcNAc transferase I, GlcNAc-T I, GlcNAc-TI, GlcNAcT-I, gly-12, GLY-13, gly-14, GnT I, GnT-I, GnT1, GnTI, Golgi N-glycan branching N-acetylglucosaminyltransferase I, HuGnTI, MGAT1, N-acetylglucosaminyltransferase I, N-acetylglucosaminyltransferases I, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, NT, pigGnT-I, PtGnTI, TbGnTI, UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase, UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, UDP-GlcNac:alpha3-D-mannoside beta1,2-Nacetylglucosaminyltransferase I, UDP-GlcNAc:alpha3-D-mannoside beta1-2-N-acetylglucosaminyltransferase I, UDP-N-acetyl-D-glucosamine:glycoprotein (N-acetyl-D-glucosamine to alpha-D-mannosyl-1,3-(R1)-beta-D-mannosyl-R2) beta-1,2-N-acetyl-D-glucosaminyltransferase, UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.101 alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

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Results	in table
9	Activating Compound
2690	AA Sequence
5	Application
1	CAS Registry Number
32	Cloned(Commentary)
1	Crystallization (Commentary)
42	Disease/ Diagnostics
3	Expression
36	General Information
4	General Stability
28	Inhibitors
9	Ki Value [mM]
52	KM Value [mM]
45	Localization
82	Metals/Ions
31	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
71	Organism
5	Pathway
7	PDB ID
15	pH Optimum
4	pH Range
1	pH Stability
6	Posttranslational Modification
40	Protein Variants
18	Purification (Commentary)
10	Reaction
1	Reaction Type
68	Reference
47	Source Tissue
15	Specific Activity [micromol/min/mg]
6	Storage Stability
183	Substrates and Products (Substrate)
10	Subunits
112	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
2	Temperature Range [°C]
1	Temperature Stability [°C]

Purification

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Purification on EC 2.4.1.101 - alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

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2 entries

affinity chromatography

Sus scrofa

488361

amylose resin column chromatography, gel filtration

Nicotiana tabacum

Q9ST97

722834

antibody purification from homogenized transfected Nicotiniana benthamiana leaves, resuspended in extraction buffer (PBS, pH 7.2, 1% Triton-X-100, protease inhibitor cocktail), centrifugation, supernatant incubated with rProtein A-Sepharose, elution by boiling Laemmli sample buffer, protein gel blot analysis with specific antibodies

Arabidopsis thaliana

710638

large scale, recombinant maltose-binding fusion protein

liver

mutant enzyme D144N

one Ni2+-NTA chromatography step is sufficient for oligosaccharide synthesis

Homo sapiens

707016

partial

2 entries

recombinant catalytic fragment from Sf9 insect cells

Oryctolagus cuniculus

P27115

488376

recombinant glutathione S-transferase fusion isozymes and mutants from insect Sf21 cells

Xenopus laevis

488381

recombinant N-terminally His-tagged protein from Escherichia coli

Rattus norvegicus

488372

two forms: transferase A and B, the latter only in hepatoma

Rattus norvegicus

488363

two molecular weight forms separable by gel filtration, purification of low-molecular weight form

Oryctolagus cuniculus

488368