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Literature summary for 2.4.1.101 extracted from

  • Oppenheimer, C.L.; Hill, R.L.
    Purification and characterization of a rabbit liver alpha 1-3 mannoside beta 1-2 N-acetylglucosaminyltransferase (1981), J. Biol. Chem., 256, 799-804.
    View publication on PubMed

General Stability

General Stability Organism
repeated freeze-thawing, stable to Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 3 mM Oryctolagus cuniculus
additional information high ionic strength, e.g. 0.1 M NaCl Oryctolagus cuniculus
UDP
-
Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ no activation Oryctolagus cuniculus
Ca2+ slight activation Oryctolagus cuniculus
Cd2+ activation Oryctolagus cuniculus
Cd2+ 78% efficiency Oryctolagus cuniculus
Co2+ activation Oryctolagus cuniculus
Co2+ 91% efficiency Oryctolagus cuniculus
Fe2+ activation Oryctolagus cuniculus
Fe2+ slight Oryctolagus cuniculus
Mg2+ activation Oryctolagus cuniculus
Mg2+ 61% efficiency Oryctolagus cuniculus
Mn2+ requirement Oryctolagus cuniculus
additional information no activation by Cu2+ Oryctolagus cuniculus
additional information no activation by Hg2+ Oryctolagus cuniculus
Ni2+ activation Oryctolagus cuniculus
Ni2+ 34% efficiency Oryctolagus cuniculus
Zn2+ activation Oryctolagus cuniculus
Zn2+ 19% efficiency Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
two MW-species Oryctolagus cuniculus
46000
-
x * 58000 + x * 46000, SDS-PAGE Oryctolagus cuniculus
58000
-
x * 58000 + x * 46000, SDS-PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.51
-
purified enzyme Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol v/v, up to a year Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
Oryctolagus cuniculus ?
-
?
UDP-N-acetyl-alpha-D-glucosamine +
-
Oryctolagus cuniculus ?
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R substrate specificity Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
UDP-N-acetyl-D-glucosamine + dehexoso-orosomucoid
-
Oryctolagus cuniculus ?
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetylglucosamine-1,4-(fucose-1,6)-N-acetyl-glucosamine-Asn-peptide no activity Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetylglucosamine-1,4-(fucose-1,6)-N-acetyl-glucosamine-Asn-peptide
-
?

Subunits

Subunits Comment Organism
? x * 58000 + x * 46000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 6.7
-
Oryctolagus cuniculus