Cloned (Comment) | Organism |
---|---|
cloning of isozymes A and B, 2 separate genes, DNA sequence determination and analysis | Xenopus laevis |
functional expression of isozymes A and B in Spodoptera frugiperda Sf21 cells as glutathione-S-transferase fusion proteins via baculovirus infection, isozyme B is folded less efficiently | Xenopus laevis |
Protein Variants | Comment | Organism |
---|---|---|
T223A | isozyme B, improvement of properties of isozyme B to the level of isozyme A | Xenopus laevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
pyridylaminated Man3GlcNAc2 | recombinant isozyme A | Xenopus laevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | medial enzyme | Xenopus laevis | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
type II membrane proteins: short N-terminal cytoplasmic tail of 4 amino acid residues, transmembrane domain of 22 residues, stem region of 81 residue for isozyme A and 77 residues for isozyme B, amino acid sequences alignment | Xenopus laevis |
65000 | - |
x * 65000, recombinant isozyme B, SDS-PAGE | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | Xenopus laevis | key enzyme of biosynthesis of complex and hybrid N-glycans | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
- |
- |
Xenopus laevis | - |
2 isozymes A and B | - |
Purification (Comment) | Organism |
---|---|
recombinant glutathione S-transferase fusion isozymes and mutants from insect Sf21 cells | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | isozyme B, low content of isozyme A | Xenopus laevis | - |
kidney | isozyme B, low content of isozyme A | Xenopus laevis | - |
liver | isozymes A and B | Xenopus laevis | - |
muscle | isozymes A and B | Xenopus laevis | - |
ovary | isozymes A and B | Xenopus laevis | - |
SF-21 cell | - |
Spodoptera frugiperda | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
recombinant isozyme A | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP + pyridylaminated Man3GlcNAc2 | - |
Xenopus laevis | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | no activity with pyridylaminated substrate | Spodoptera frugiperda | UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | recombinant isozyme A and B mutant T223A | Xenopus laevis | UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | pyridylamine | Xenopus laevis | UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | key enzyme of biosynthesis of complex and hybrid N-glycans | Xenopus laevis | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
Xenopus laevis | UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, recombinant isozyme B, SDS-PAGE | Xenopus laevis |
Synonyms | Comment | Organism |
---|---|---|
GnT-I | - |
Xenopus laevis |
UDP-N-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I | - |
Xenopus laevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
assay at | Xenopus laevis |