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2.3.2.2: gamma-glutamyltransferase

This is an abbreviated version!
For detailed information about gamma-glutamyltransferase, go to the full flat file.

Word Map on EC 2.3.2.2

Reaction

a (5-L-glutamyl)-peptide
+
an amino acid
=
a peptide
 +
a 5-L-glutamyl amino acid

Synonyms

(5-L-glutamyl) peptide: amino-acid 5-glutamyl transferase, alpha-glutamyl transpeptidase, AngammaGT, AsGGT1, At1g69820, At4g29210, At4g39640, At4g39650, BaGGT, BaGGT42, BaGGT469, BlGGT, BlGGT13, BsGGT168, Cgl0954, eqGGT, gamma glutamyl transferase, gamma-glutamyl peptidyltransferase, gamma-glutamyl transferase, gamma-glutamyl transferase 1, gamma-glutamyl transferase 5, gamma-glutamyl transferase/transpeptidase, gamma-glutamyl transferases, gamma-glutamyl transpeptidase, gamma-glutamyl transpeptidase 1, gamma-glutamyl transpeptidase 4, gamma-glutamyl transpeptidase-1, gamma-glutamyl-transpeptidase, gamma-glutamyltransferase, gamma-glutamyltranspeptidase, gamma-GPT, gamma-GT, gamma-GTase, gamma-GTP, gammaGT, GGT, GGT A, GGT I, GGT-1, GGT1, GGT2, GGT3, GGT4, GGT5, ggtB, GGTII protein, GGTLA1, glutamyl transpeptidase, glutamyltransferase, gamma-, HGGT, L-gamma-glutamyl transpeptidase, L-gamma-glutamyltransferase, L-glutamyltransferase, More, PnGGT, SBLGGT, VvGGT3

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.2 gamma-glutamyltransferase

Cloned

Cloned on EC 2.3.2.2 - gamma-glutamyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a truncated Escherichia coli Novablue gamma-glutamyltranspeptidase gene lacking the first 48-bp coding sequence for part of the signal sequence is amplified by polymerase chain reaction and cloned into expression vector pQE-30 to generate pQE-EcGGT. The maximum production of His6-tagged enzyme by Escherichia coli M15 is achieved with 0.1 mM IPTG induction for 12 h at 20°C
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cloning of liver specific isoform III from fetal mRNA, screening of rat genetic library, single copy gene, DNA, including enhancer and promotor, sequence determination, plasmid construction and expression in Escherichia coli, transient enzyme expression in hepatoma cell lines, dependent on differentiation state of the cells
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expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli M15 cells
expressed in Pichia pastoris
expressed in Pichia pastoris and in HEK-293 cells
expression in CHO cells
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expression in Escherichia coli
expression in mouse fibroblast
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expression of His6-tagged enzyme in Escherichia coli M15
expression of mutant enzymes in Escherichia coli
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expression of recombinant wild-type and mutants in Spodoptera frugiperda Sf21 cells via baculovirus infection
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expression of wild-type and mutant lacking the putative signal sequence/anchor domain, amino acid 1-27, in Spodoptera frugiperda Sf21 cells via baculovirus infection
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full-length coding region
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gene BaGGT469, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Bacillus subtilis strain KCTC 3135 and in Escherichia coli strain BL21(DE3)
gene BaGGT469, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene blggt, recombinant overexpression of extracellular enzyme in Escherichia coli strain BL21(DE3) in bioreactor under batch and fed-batch condition, development and optimization of a cost-effective method for overexpression and preparation of bacterial GGT enzyme for its industrial applications, method optimization, overview. The native signal is used for extracellular expression of the enzyme in Escherichia coli. Enzyme activity of 24500 U/l in the extracellular medium after 24 h. Subcloning in Escherichia coli strain DH5alpha, expression studies in Escherichia coli strains M!5 and BL21(DE3)
gene BsGGT168, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ggt, recombinant expression of His-tagged enzyme GGT in Escherichia coli strain M15
gene ggt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)-pET22b
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gene ggt, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain M15, subcloning in Escherichia coli strain XL-1 Blue
gene ggt, recombinant expression of His6-tagged enzyme in Bacillus subtilis strain 168 (pHT43-spAmyQ-ggtDELTAsp) under control of the Plac promoter, extracellular secretion, subcloning in Escherichia coli strain DH5alpha, method optimization. Dual-signal peptides promote more secretion than a single signal peptide
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gene ggt, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain M15, the recombinant BlGGT confers the ability to catalyze the synthesis of gamma-L-glutamyl-S-allyl-L-cysteine, gamma-glutamyl-L-phenylalanine, and gamma-glutamyl-L-leucine
gene ggt, recombinant expression of wild-type and K562S mutant enzymes in Spodoptera frugiperda Sf9 cells via baculovirus transfection method
gene ggt, recombinant overexpression of wild-type and mutant enzymes
gene ggt, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15
Q62WE3
gene ggt, sequence comparisons, recombinant expression of Strep-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene GGT1, recombinant expression of enzyme mutant V272A in Pichia pastoris strain X-33
gene GGT3, Vitis vinifera appears to contain only a single GGT gene, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, transient functional recombinant overexpression of GFP-tagged enzyme in Allium cepa cells and in Nicotiana benthamiana leaves via transformation with Agrobacterium tumefaciens strain GV3101, quantitative RT-PCR exxpression analysis
gene ggtB, recombinat expression of GgtB with an N-terminal fusion of the chitin-binding domain and intein tag to the target protein, subcloning in Escherichia coli strain DH5alphaMCR and subsequently transformation into and expression in Escherichia coli strain ER25669
generation of transgenic mice that overexpress gamma-glutamyltransferase in a tissue-specific manner utilizing the Cre-loxP recombination system. Systemic as well as local production of gamma-glutamyltransferase accelerates osteoclast development and bone resorption in vivo by increasing the sensitivity of bone marrow macrophages to receptor activator of nuclear factor-kappaB ligand, an essential cytokine for osteoclastogenesis. Mutated gamma-glutamyltransferase devoid of enzyme activity is as potent as the wild-type molecule in inducing osteoclast formation, suggesting that gamma-glutamyltransferase acts not as an enzyme but as a cytokine
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GGT of Bacillus subtilis is synthesized only during the mid-stationary phase, which is inconvenient for industrial use, a strain overexpressing GGT for a sufficiently long period is generated to obtain large quantities of GGT. A plasmid vector, pHY300PLK, containing the ggt gene cloned from chromosomal DNA is introduced into a spo0A abrB double mutant strain, in which the level of GGT activity is high after the mid-stationary phase. The level of GGT activity in this strain increases steadily after the exponential phase, becoming 15fold higher than that in the parental strain
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overexpression as His-tagged protein in Escherichia coli JM109, DNA sequence analysis
O65652, Q39078, Q9M0G0
recombinant expression of His-tagged wild-type and mutant GGTs in Escherichia coli strain BL21(DE3), recombinant overexpression of GGT in Bacillus subtilis strain MH2308 from plasmid pMH2312, semi-quantitative reverse-transcription PCR enzyme expression analysis
the enzyme is overexpressed under the control of a constitutive Histoplasma capsulatum-native promoter
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the structural gene encoding GGTII is amplified from the genomic DNA of the fission yeast and ligated into the shuttle vector pRS316 to generate the recombinant plasmid pPHJ02. The Schizosaccharomyces pombe cells harboring plasmid pPHJ02 show about 4fold higher GGT activity in the exponential phase than cells harboring the vector only
transfection of human melanoma cell
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transfection of Me665/2 cell
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transfection of Me665/2/21 human melanoma cell, DU145 human prostatic carcinoma cell, and BEAS-2B human bronchial epithelial cell
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