Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
in complex with L-glutamate, hanging drop vapor diffusion method, using poly(ethylene glycol) 4000, 100 mM MES buffer (pH 7.0), 600 mM NaCl, and 5% (v/v) Jeffamine M-600 | Bacillus subtilis |
General Stability | Organism |
---|---|
the enzyme retains 86% of its activity even in 3 M NaCl | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 40000 + 1 * 20000, SDS-PAGE | Bacillus subtilis |
40000 | - |
1 * 40000 + 1 * 20000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P54422 | - |
- |
Bacillus subtilis 168 | P54422 | - |
- |
Purification (Comment) | Organism |
---|---|
N-terminal His-tagged enzyme is purified by ammonium sulfate precipitation and Sephacryl S-200 gel filtration | Bacillus subtilis |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 40000 + 1 * 20000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyltranspeptidase | - |
Bacillus subtilis |
GGT | - |
Bacillus subtilis |