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Literature summary for 2.3.2.2 extracted from
- Purification and cloning of a gamma-glutamyl transpeptidase from onion (Allium cepa) (2005), Phytochemistry, 66, 515-522.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length coding region | Allium cepa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-carboxypropylglutathione | - |
Allium cepa |  |
| gamma-glutamyl trans-S-1-propenyl cysteine sulfoxide | uncompetitive | Allium cepa | |
| GSH | - |
Allium cepa | |
| S-methyl glutathione | - |
Allium cepa | |
| S-propyl glutathione | - |
Allium cepa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
5-L-glutamyl-4-nitroanilide | - |
Allium cepa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated to | Allium cepa | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium cepa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Allium cepa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | sprouting | Allium cepa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.29 | - |
isoform I | Allium cepa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-L-glutamyl-4-nitroanilide + H2O | - |
Allium cepa | 4-nitroaniline + Glu | - |
? | |
| 5-L-glutamyl-4-nitroanilide + L-Met | - |
Allium cepa | 4-nitroaniline + 5-L-glutamyl-L-Met | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GGT | - |
Allium cepa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
transpeptidation | Allium cepa |
| 9 | - |
hydrolysis | Allium cepa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0164 | - |
GSH | - |
Allium cepa | |
| 0.0233 | - |
S-methyl glutathione | - |
Allium cepa | |
| 0.0679 | - |
S-propyl glutathione | - |
Allium cepa | |
| 0.1137 | - |
2-carboxypropylglutathione | - |
Allium cepa | |
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