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Literature summary for 2.3.2.2 extracted from

  • Balakrishna, S.; Prabhune, A.
    Effect of pH on the hydrolytic kinetics of gamma-glutamyl transferase from Bacillus subtilis (2014), ScientificWorldJournal, 2014, 216270.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16.7
-
 glutamyl-(3-carboxyl)-4-nitroanilide at pH 9.0 and 30°C Bacillus subtilis
25.9
-
 glutamyl-(3-carboxyl)-4-nitroanilide at pH 7.5 and 30°C Bacillus subtilis
26.9
-
 glutamyl-(3-carboxyl)-4-nitroanilide at pH 11.0 and 30°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 45000 + 1 * 22000, SDS-PAGE Bacillus subtilis
45000
-
 1 * 45000 + 1 * 22000, SDS-PAGE Bacillus subtilis
66000
-
 gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
-
 Bacillus subtilis 3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 45000 + 1 * 22000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
gamma glutamyl transferase
-
 Bacillus subtilis
GGT
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7 11 the enzyme is active between pH 7.0 and 11.0 Bacillus subtilis