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2.3.2.2: gamma-glutamyltransferase

This is an abbreviated version!
For detailed information about gamma-glutamyltransferase, go to the full flat file.

Word Map on EC 2.3.2.2

Reaction

a (5-L-glutamyl)-peptide
+
an amino acid
=
a peptide
 +
a 5-L-glutamyl amino acid

Synonyms

(5-L-glutamyl) peptide: amino-acid 5-glutamyl transferase, alpha-glutamyl transpeptidase, AngammaGT, AsGGT1, At1g69820, At4g29210, At4g39640, At4g39650, BaGGT, BaGGT42, BaGGT469, BlGGT, BlGGT13, BsGGT168, Cgl0954, eqGGT, gamma glutamyl transferase, gamma-glutamyl peptidyltransferase, gamma-glutamyl transferase, gamma-glutamyl transferase 1, gamma-glutamyl transferase 5, gamma-glutamyl transferase/transpeptidase, gamma-glutamyl transferases, gamma-glutamyl transpeptidase, gamma-glutamyl transpeptidase 1, gamma-glutamyl transpeptidase 4, gamma-glutamyl transpeptidase-1, gamma-glutamyl-transpeptidase, gamma-glutamyltransferase, gamma-glutamyltranspeptidase, gamma-GPT, gamma-GT, gamma-GTase, gamma-GTP, gammaGT, GGT, GGT A, GGT I, GGT-1, GGT1, GGT2, GGT3, GGT4, GGT5, ggtB, GGTII protein, GGTLA1, glutamyl transpeptidase, glutamyltransferase, gamma-, HGGT, L-gamma-glutamyl transpeptidase, L-gamma-glutamyltransferase, L-glutamyltransferase, More, PnGGT, SBLGGT, VvGGT3

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.2 gamma-glutamyltransferase

Purification

Purification on EC 2.3.2.2 - gamma-glutamyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoforms I and II
-
enzyme form GGT A
-
enzyme form GGT B
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enzyme form GGT I
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enzyme form GGT II
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from carcinoma and partially from normal mammary gland
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from hyperplastic hepatic nodules, Yoshida ascites hepatoma, primary hepatoma, kidney
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from normal and cirrhotic liver and 6 isoforms from serum of patients with cellular hepatic carcinoma
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from normal and diabetic rat liver
-
from secretion
-
from seminal plasma, kidney, prostate, and testis
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HisTrap column chromatography and Superdex S200 gel filtration
-
Lubrol WX/deoxycholate
-
mutant enzymes from Escherichia coli
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N-terminal His-tagged enzyme is purified by ammonium sulfate precipitation and Sephacryl S-200 gel filtration
native enzyme 40.7fold from leaves by ammonium sulfate fractionation, dialysis, and gel filtration
-
native enzyme from bulbs by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration
native extracellular enzyme 4.6fold from Bacillus licheniformis strain ER15 by ultrafiltration, gel filtration, ion exchange chromatography, and dialysis, with 50.11% yield
native extracellular enzyme partially from carbon stressed cultures
-
Ni2+-NTA resin column chromatography
partial
partial from bile
-
partial from ciliary body, solubilized with Emulphogene BC720
-
partial from liver
-
partial from liver, highly from biliary tract
-
partial from mammary-tumour virus and from milk fat-globule membranes, in presence of Triton X-100
-
partial from pancreas and pancreatic carcinoma and pancreatic carcinoma cell line HPC-Y1
-
partial, 3 isoforms
-
Q-Sepharose column chromatography and G-75 Sephadex gel filtration
-
recombinant
recombinant enzyme
recombinant extracellular C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant from E. coli
-
recombinant GGT1 mutant V272A from Pichia pastoris strain X-33
recombinant His-tagged enzyme from E. coli
O65652, Q39078, Q9M0G0
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-pET22b by nickel affinity chromatography and dialysis
-
recombinant His-tagged GGT from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography
Q62WE3
recombinant His-tagged wild-type and mutant GGT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged eextracellular enzyme from Bacillus subtilis strain 168 by nickel affinity chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain M15 to near homogeneity by nickel affinity chromatography
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography and ultrafiltration, to homogeneity
recombinant wild-type and mutant enzymes
recombinant wild-type and mutants from Sf21 insect cells
-
recombinat GgtB N-terminally tagged with chitin-binding domain and intein tag from Escherichia coli strain ER25669 by chitin affinity chromatography and ultrafiltration
salt fractionation and gel filtration on Biogel P-10 and P-200
-
solubilized by deoxycholate
-
solubilized by Triton X-100
-
solubilized with either detergents or proteinases
-
solubilized with ficin
-
solubilized with Lubrol/deoxycholate
-
Triton X-100 solubilized liver enzyme is hydrophobic, papain-solubilized liver enzyme is hydrophilic
-