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2.2.1.6: acetolactate synthase

This is an abbreviated version!
For detailed information about acetolactate synthase, go to the full flat file.

Word Map on EC 2.2.1.6

Reaction

2 pyruvate =

2-acetolactate
+
CO2

Synonyms

aceto-hydroxy acid synthase, acetohydroxy acid synthase, acetohydroxy acid synthase I, acetohydroxy acid synthetase, acetohydroxyacid synthase, acetolactate pyruvate-lyase (carboxylating), acetolactate synthase, acetolactate synthetase, acetolactate-synthase, acetolactic synthetase, AHAS, AHAS II, AHAS1, AHAS2, AHAS3, AHASL, AHASS, alpha-acetohydroxy acid synthetase, alpha-acetohydroxyacid synthase, alpha-acetolactate synthase, alpha-acetolactate synthetase, alpha-ALS, ALS, ALS1, ALS1R, ALS2, AlsS, CalS, catabolic acetolactate synthase, EC 4.1.3.18, GST-mALS, GST-wALS, Ilv2, Ilv6, ilvB, ilvB1, ilvB2, ilvG, IlvN, ilvX, Moilv2, Moilv6, More, PF0935, sll1981, synthase, acetolactate, TM_0548, TM_0549, TTHA1213

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.6 acetolactate synthase

Purification

Purification on EC 2.2.1.6 - acetolactate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Ni-NTA-Sefinose column chromatography
-
ammonium sulfate precipitation and Sephadex G-25 gel filtration
-
DEAE-Sepharose column chromatography and hydroxyapatite column chromatography
gel filtration
-
His-tagged recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to 96% purity
-
isoenzyme II
-
isoenzyme II, recombinantly produced
-
mutant enzymes A122V, W574S, W574L and S653N expressed in Escherichia coli
-
native enzyme to homogeneity
-
Ni+-chelating column chromatography
Ni-NTA column chromatography
Ni-NTA resin column chromatography and S-200 HR gel filtration
Ni-Sepharose column chromatography
Ni2+-NTA column chromatography, HiTrap Q column chromatography, and Superdex S200 gel filtration
partial
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, to homogeneity
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant catalytic and regulatory subunits from Escherichia coli strain BL21(DE3) Rosetta 2, the catalytic subunit is purified 30.2fold by heat treatement, and anion exchange and hydroxyapatite chromatography
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM105 by glutathione affinity chromatography
recombinant His-tagged AHAS catalytic subunit from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography, and gel filtration
recombinant His-tagged catalytic subunit to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinit chromatography and gel filtration
recombinant His-tagged enzyme subunits from Escherichia coli to homogeneity by nickel affinity chromatography and dialysis
-
recombinant His-tagged isozyme I holoenzyme or individual subunits from strain BL21 by affinity chromatography
-
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant regulatory subunit from Escherichia coli strain BL21(DE3) Rosetta 2, the regulatory subunit is purified to homogeneity by heat treatment at 80°C for 1h and nickel affinity chromatography
recombinant soluble His-tagged enzyme mutants from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography, gel filtration, and anion exchange chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain DH5alpha
-
recombinant wild-type and mutant enzymes from Escherichia coli strains JM109 and Rosetta 2 (DE3) partially by heat treatment at 50°C or 70°C for 30 min
recombinant wild-type and mutant GST-tagged enzymes from Escherichia coli strain BL21(DE3) by two steps of glutathione affinity chromatography to homogeneity
-
recombinant wild-type and mutants enzymes from Escherichia coli strain BL21(DE3)
-
wild-type and natural mutant enzymes from rice leaves