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2.2.1.6: acetolactate synthase

This is an abbreviated version!
For detailed information about acetolactate synthase, go to the full flat file.

Word Map on EC 2.2.1.6

Reaction

2 pyruvate =

2-acetolactate
+
CO2

Synonyms

aceto-hydroxy acid synthase, acetohydroxy acid synthase, acetohydroxy acid synthase I, acetohydroxy acid synthetase, acetohydroxyacid synthase, acetolactate pyruvate-lyase (carboxylating), acetolactate synthase, acetolactate synthetase, acetolactate-synthase, acetolactic synthetase, AHAS, AHAS II, AHAS1, AHAS2, AHAS3, AHASL, AHASS, alpha-acetohydroxy acid synthetase, alpha-acetohydroxyacid synthase, alpha-acetolactate synthase, alpha-acetolactate synthetase, alpha-ALS, ALS, ALS1, ALS1R, ALS2, AlsS, CalS, catabolic acetolactate synthase, EC 4.1.3.18, GST-mALS, GST-wALS, Ilv2, Ilv6, ilvB, ilvB1, ilvB2, ilvG, IlvN, ilvX, Moilv2, Moilv6, More, PF0935, sll1981, synthase, acetolactate, TM_0548, TM_0549, TTHA1213

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.6 acetolactate synthase

Cloned

Cloned on EC 2.2.1.6 - acetolactate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ALS gene, DNA and amino acid sequence determination and analysis, expression of GST-tagged ALS wild-type and mutant enzymes in Escherichia coli strain JM105. expression in transgenic Oryza sativa var. Nipponbare plants using the Agrobacterium tumefaciens transfection method
cloned into the bacterial expression plasmid pKK233-2, expression in AHAS-deficient Escherichia coli strain MF2000
-
cloned into the bacterial expression vector pGEX-2T and used to transform Escherichia coli strain pGEX-2T
-
cloned into the bacterial expression vector pT7-7 and expressed in Escherichia coli BL21(DE3)
-
co-expression of acetolactate synthase and omega-transaminase in Escherichia coli
-
DNA and amino acid sequence determination and analysis, genotyping
-
expressed in Corynebacterium glutamicum wild type strain ATCC14067 and its mutant YTW-104
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) Rosetta 2 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLys Express and HMS174(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in Klebsiella pneumoniae KG-rs cells
-
expressed in Oryza sativa subsp. japonica variety JD164 and Escherichia coli Rosetta cells
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli strain DH5alpha
-
expression in Glycine max
-
expression is considerably higher from a vector that introduces a 50 residue N-terminal fusion containing an oligohistidine sequence on the large subunit
-
expression of the His-tagged catalytic subunit
-
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
-
expression of wild-type and mutant enzymes via shuttle vector pECKA in Escherichia coli and in Corynebacterium glutamicum
-
expression of wild-type and mutant GST-tagged enzymes in Escherichia coli strain BL21(DE3)
-
gene AHAS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), the enzyme contains additional 34 residues, MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS, at the N-terminus
-
gene ahas1 encoding the AHAS catalytic subunit, DNA and amino acid sequence determination and analysis, expression pattern of ahas genes family in sunflower
gene ahas2 encoding the AHAS catalytic subunit, DNA and amino acid sequence determination and analysis, expression pattern of ahas genes family in sunflower
gene ahas3 encoding the AHAS catalytic subunit, DNA and amino acid sequence determination and analysis, expression pattern of ahas genes family in sunflower
gene als or PF0935, DNA and amino acid sequence determination and analysis
gene als, amino acid sequence determination and analysis, sequence comparisons
gene als, cloning in Escherichia coli strain DH5alpha
-
gene als, DNa and amino acid sequence determination and analysis
gene ALS, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, the ALS gene exists as a single copy gene in rice and contains no introns
gene als, genetic location within the butanediol operon, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene als, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene als, recombinant expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and Rosetta 2 (DE3)
gene ALS, recombinant exxpression of N-terminally His6-tagged wild-type and mutant enzymes with a 85 amino acid N-terminal deletion eliminating the chloroplast transit peptide
gene ALS1, DNA and amino acid sequence determination and analysis
gene ALS2, DNA and amino acid sequence determination and analysis
gene ALSa, DNA and amino acid sequence determination and analysis, genotyping, expression analysis
gene ALSb, DNA and amino acid sequence determination and analysis, genotyping, expression analysis
gene alsS, inducible overexpression in a constructed pta mutant strains RH35 and RH36 of Bacillus subtilis, the recombinant overexpression of the enzyme leads to increased acetolactate synthase activity and alteration of carbon flux into the acetoin biosynthesis pathway, alterations of involved enzyme activities, overview
-
gene ilvB, DNA and amino acid sequence determination and analysis
gene ilvH, encoding the regulatory subunit SSU, expression of wild-type and mutants, and of the selenomethionine variant
-
gene yerE, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
genes MGG_01104 and MGG_06868, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of GFP-tagged subunits in the deletion mutant strains, subcellular localization of both MoIlv2 and MoIlv6 to the mitochondria
G4NC97; G4MMK2
GST-wALS, and sulfonylurea-resistant enzyme fused with glutathione S-transferase, GST-mALS
-
His-tagged AHAS catalytic subunit , overexpression in Escherichia coli strain BL21(DE3)
inducible overexpression of the His-tagged enzyme subunits in Escherichia coli strain BL21(DE3)
-
isozyme ALS1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetics, genotyping
-
isozyme I subunit-encoding genes ilvB and ilvN, DNA and amino acid sequence determination and analysis, expression of His-tagged holoenzyme or individual subunits in strain BL21
-
overexpression in Escherichia coli
-
phylogenetic analysis, recombinant expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
recombinant expression of the catalytic subunit in Escherichia coli strain BL21 (DE3) Rosetta 2, subcloning in Escherichia coli strain DH5alpha, reconstitution of the catalytic and regulatory subunits leads to increased AHAS activity
recombinant expression of the regulatory subunit in Escherichia coli strain BL21 (DE3) Rosetta 2, subcloning in Escherichia coli strain DH5alpha, reconstitution of the catalytic and regulatory subunits leads to increased AHAS activity
recombinant expression of the wild-type enzyme's catalytic subunit, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type and mutants enzymes in Escherichia coli strain BL21(DE3)
-
sequence comparisoons, recombinant expression of soluble His-tagged enzyme mutants in Escherichia coli strain BL21(DE3)
sll1981, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning and expression in Escherichia coli and in Schizosaccharomyces pombe strain PR 109, functional complementation of a Schizosaccharomyces pombe mutant strain
-
wild-type recombinant enzyme fused with glutathione S-transferase
-