Protein Variants | Comment | Organism |
---|---|---|
A122V | reduced affinity for all Mg2+, thiamine diphosphate and FAD | Arabidopsis thaliana |
S653N | binds FAD more strongly than the wild-type enzyme | Arabidopsis thaliana |
W574L | insensitive to sulfonurea herbicides | Arabidopsis thaliana |
W574S | reduction in sensitivity to sulfonurea herbicides compared to the wild-type enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Arabidopsis thaliana | first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
mutant enzymes A122V, W574S, W574L and S653N | - |
Purification (Comment) | Organism |
---|---|
mutant enzymes A122V, W574S, W574L and S653N expressed in Escherichia coli | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.4 | - |
mutant enzyme W574L | Arabidopsis thaliana |
8.51 | - |
mutant S653N | Arabidopsis thaliana |
11 | - |
mutant W574 | Arabidopsis thaliana |
12 | - |
mutant enzyme A122V | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-70°C, 21 days, 15% loss of activity of mutant enzyme A122V, less than 1% loss of activity of mutant enzymes W574S and S653N, 3% loss of activity of mutant enzyme W574L | Arabidopsis thaliana |
4°C, 21 days, 30% loss of activity of mutant enzyme A122V, 6% loss of activity of mutant enzyme W574S, 20% loss of activity of mutant enzyme W574L, 18% loss of activity of mutant enzyme S653N | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Arabidopsis thaliana | 2-acetolactate + CO2 | - |
? | |
pyruvate | first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile | Arabidopsis thaliana | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
enzyme from mutant A122V, W574S, W574L and S653N | Arabidopsis thaliana |