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Literature summary for 2.2.1.6 extracted from
- Characterization of recombinant FAD-independent catabolic acetolactate synthase from Enterococcus faecalis V583 (2013), Enzyme Microb. Technol., 52, 54-59.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by FAD | Enterococcus faecalis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Enterococcus faecalis |
| gene als, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Enterococcus faecalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics and optimal reaction conditions, overview | Enterococcus faecalis | |
| 1.37 | - |
pyruvate | pH 6.8, 37°C, recombinant enzyme | Enterococcus faecalis |  |
| 1.37 | - |
pyruvate | at pH 6.8 and 37°C | Enterococcus faecalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Enterococcus faecalis | |
| Mg2+ | independent | Enterococcus faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Enterococcus faecalis |
| 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Enterococcus faecalis |
| 60200 | - |
x * 60200, calculated from amino acid sequence | Enterococcus faecalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 pyruvate | Enterococcus faecalis | - |
2-acetolactate + CO2 | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | the recombinant enzyme shows a loss of over 50% activity | Enterococcus faecalis |
| Acetone | the activity decreases to less than 50% in the presence of 15% (v/v) acetone | Enterococcus faecalis |
| DMSO | the recombinant enzyme is extremely stable in DMSO | Enterococcus faecalis |
| DMSO | the enzyme exhibits extreme stability in the presence of up to 20% (v/v) DMSO | Enterococcus faecalis |
| Ethanol | the recombinant enzyme shows a loss of over 50% activity | Enterococcus faecalis |
| Ethanol | the activity decreases to less than 50% in the presence of 15% (v/v) ethanol and to 7% at 20% (v/v) ethanol | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | Q836A5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Enterococcus faecalis |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinit chromatography and gel filtration | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 pyruvate | - |
Enterococcus faecalis | 2-acetolactate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Enterococcus faecalis |
| ? | x * 60000, recombinant enzyme, SDS-PAGE | Enterococcus faecalis |
| ? | x * 60200, calculated from amino acid sequence | Enterococcus faecalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALS | - |
Enterococcus faecalis |
| CalS | - |
Enterococcus faecalis |
| catabolic acetolactate synthase | - |
Enterococcus faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Enterococcus faecalis |
| 37 | - |
recombinant enzyme | Enterococcus faecalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the activity above 40°C is less than 20% and then completely decreases at higher temperatures | Enterococcus faecalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
pyruvate | at pH 6.8 and 37°C | Enterococcus faecalis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Enterococcus faecalis |
| 6.8 | - |
recombinant enzyme | Enterococcus faecalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not FAD-dependent | Enterococcus faecalis | |
| thiamine diphosphate | - |
Enterococcus faecalis | |
| thiamine diphosphate | dependent on | Enterococcus faecalis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
pyruvate | at pH 6.8 and 37°C | Enterococcus faecalis | |
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