2.1.1.13: methionine synthase

This is an abbreviated version!
For detailed information about methionine synthase, go to the full flat file.

Reaction

Synonyms

5-methyltetrahydrofolate homocysteine methyltransferase, 5-methyltetrahydrofolate-homocysteine methyltransferase, 5-methyltetrahydrofolate-homocysteine S-methyltransferase, 5-methyltetrahydrofolate-homocysteine transmethylase, B12 N5-methyltetrahydrofolate homocysteine methyltransferase, B12-dependent methionine synthase, cobalamin-dependent methionine synthase, folate-dependent methionine synthase, Met6p, MetH, methionine synthase, methionine synthetase, methyltetrahydrofolate-homocysteine vitamin B12 methyltransferase, methyltransferase, methyltetrahydrofolate-homocysteine, MetS, MS, MS1, MTR, N-methyltetrahydrofolate:L-homocysteine methyltransferase, N5-methyltetrahydrofolate methyltransferase, N5-methyltetrahydrofolate-homocysteine cobalamin methyltransferase, N5-methyltetrahydrofolic-homocysteine vitamin B12 transmethylase, tetrahydrofolate methyltransferase, tetrahydropteroylglutamate methyltransferase, tetrahydropteroylglutamic methyltransferase, vitamin B12 methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.13 methionine synthase

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Results	in table
20	Activating Compound
32678	AA Sequence
10	Application
1	CAS Registry Number
10	Cloned(Commentary)
99	Cofactor
10	Crystallization (Commentary)
27596	Disease/ Diagnostics
3	Expression
3	General Information
10	General Stability
20	IC50 Value
20	Inhibitors
2	Ki Value [mM]
25	KM Value [mM]
6	Localization
10	Metals/Ions
21	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
1342	Organism
13	Pathway
32	PDB ID
7	pH Optimum
3	pH Range
3	pH Stability
23	Protein Variants
30	Purification (Commentary)
28	Reaction
1	Reaction Type
111	Reference
1	Renatured (Commentary)
70	Source Tissue
40	Specific Activity [micromol/min/mg]
8	Storage Stability
83	Substrates and Products (Substrate)
9	Subunits
40	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
4	Turnover Number [1/s]

Engineering

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Engineering on EC 2.1.1.13 - methionine synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D504A

Candida albicans

-

the mutant exhibits 4% of wild type activity

702050

D504N

Candida albicans

-

the mutant exhibits 11% of wild type activity

702050

D504S

Candida albicans

-

the mutant exhibits 7% of wild type activity

702050

D614A

Candida albicans

-

the mutant exhibits less than 2% of wild type activity

702050

D614N

Candida albicans

-

the mutant exhibits less than 2% of wild type activity

702050

D614S

Candida albicans

-

the mutant exhibits 11% of wild type activity

702050

E620Q

Candida albicans

-

the mutation has no effect on enzyme activity

702050

E620S

Candida albicans

-

the mutation has no effect on enzyme activity

702050

H128N

Candida albicans

-

the mutant is 5fold less active compared to the wild type GST fusion protein

702050

R530A

Candida albicans

-

the mutant exhibits 33% of wild type activity

702050

S448A

Candida albicans

-

the mutant exhibits 67% of wild type activity

702050

W576F

Candida albicans

-

the mutant is 3fold less active compared to the wild type GST fusion protein

702050

W576Y

Candida albicans

-

the mutant is 8fold less active compared to the wild type GST fusion protein

702050

Y527F

Candida albicans

-

the mutant exhibits 67% of wild type activity

702050

Cys310Ser

Escherichia coli

-

contains less than 0.05 equiv. of zinc, no proton release upon incubation with L-homocysteine, no enzymativc activity of holoenzyme

441209

Cys311Ser

Escherichia coli

-

contains less than 0.05 equiv. of zinc, no proton release upon incubation with L-homocysteine, no enzymativc activity of holoenzyme

441209

I690C/G743C

2 entries

I690C/G743C/Y1139F

Escherichia coli

-

the I690C/G743C MetH variant is trapped in the activation conformation

704166

Y1139F

Escherichia coli

P13009

the mutation lowers the cobalamine reduction potential from -490 mV to -540 mV

706520

D963E /K1071N

Homo sapiens

-

reduced activity

673604

additional information

2 entries

I690C/G743C

Escherichia coli

P13009

the mutant is locked in the reactivation conformation and found in both His-on and -off states

706495

I690C/G743C

Escherichia coli

-

the variant of a C-terminal fragment of MetH locks the enzyme into the activation conformation without perturbing any of the residues in the vicinity of the active site

704166

additional information

Escherichia coli

-

truncated MetH(2-649) protein, contains 0.9 equiv. of zinc, still proton release upon incubation with L-homocysteine

441209

additional information

Escherichia coli

P13009

truncated MetH(2-649) protein catalyzes partial enzymic reactions, truncated MetH(2-649) + Cys310Ala or + Cys311Ala indicate modular structure of enzyme comprising of four separate regions

441211