Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Hms174(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the 65-kDa I690C/G743C MetH fragment is crystallized by the microbatch method, using 0.2 M potassium nitrate and 20% (w/v) PEG3350 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
I690C/G743C | the mutant is locked in the reactivation conformation and found in both His-on and -off states | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P13009 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-charged HiTrap chelating column chromatography and Mono Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Escherichia coli | tetrahydropteroylmonoglutamate + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
B12-dependent methionine synthase | - |
Escherichia coli |
MetH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin B12 | - |
Escherichia coli |