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EC Number
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
Co(I) corrinoid cofactor
Co2+ corrinoid
cofactor is absent in strain N and therefore the strain is unable to dechlorinate tetrachloroethene
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; corrinoid protein; dicyano-cobalamin; dicyano-cob(III)alamin
vitamin B12
corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion
corrinoid probably noncavalently bound to enzyme
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