BRENDA - Enzyme Database show
show all sequences of 1.21.99.5

Purification, cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethylene (PCE) from Clostridium bifermentans DPH-1

Okeke, B.C.; Chang, Y.C.; Hatsu, M.; Suzuki, T.; Takamizawa, K.; Can. J. Microbiol. 47, 448-456 (2001)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
bioremediation of chloroethylenes, multiple chlorinated aliphatic molecules, in contaminated sites and water, anaerobic reductive dehalogenation of toxic xenobiotic; can play important role in breakdown of perchloroethene and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances
Paraclostridium bifermentans
Cloned(Commentary)
Commentary
Organism
-
Paraclostridium bifermentans
General Stability
General Stability
Organism
enzyme generally unstable
Paraclostridium bifermentans
enzyme oxygen sensitive
Paraclostridium bifermentans
purified enzyme not stable, 50% loss of activity at -30°C
Paraclostridium bifermentans
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
no effect, no inhibition
Paraclostridium bifermentans
Propyl iodide
1-iodopropane; 90% inhibition by mixture of propyl iodide and titanium citrate in the dark, light restores about 95% of initial activity; light-reversible inactivation in the presence of titanium(III)citrate as electron donor
Paraclostridium bifermentans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
enzyme kinetics and kinetic parameters
Paraclostridium bifermentans
0.012
-
tetrachloroethene
-
Paraclostridium bifermentans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
not an integral membrane protein but a peripheral membrane protein
Paraclostridium bifermentans
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cobalamin
cobalamin-containing enzyme; no significant effect of cyanocobalamin
Paraclostridium bifermentans
Cobalt
Co2+ corrinoid
Paraclostridium bifermentans
additional information
independent of metal ions
Paraclostridium bifermentans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, homodimer, SDS-PAGE
Paraclostridium bifermentans
35700
-
2 * 35700, homodimer, MALDI-TOF/MS
Paraclostridium bifermentans
70000
-
gel filtration
Paraclostridium bifermentans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tetrachloroethene + reduced acceptor
Paraclostridium bifermentans
-
trichloroethene + chloride + acceptor
-
-
?
tetrachloroethene + reduced acceptor
Paraclostridium bifermentans DPH-1
-
trichloroethene + chloride + acceptor
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paraclostridium bifermentans
-
DPH-1
-
Paraclostridium bifermentans DPH-1
-
DPH-1
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
-
Paraclostridium bifermentans
Purification (Commentary)
Commentary
Organism
-
Paraclostridium bifermentans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.086
-
-
Paraclostridium bifermentans
Storage Stability
Storage Stability
Organism
-30°C, purified enzyme not stable, 50% loss of activity
Paraclostridium bifermentans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,1,2-trichloroethane + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
1,1-dichloroethylene + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
1,2-dichloropropane + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
cis-1,2-dichloroethylene + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
additional information
substrate specificity
349709
Paraclostridium bifermentans
?
-
-
-
-
additional information
broad substrate specificity, degrades various chlorinated aliphatic compounds
349709
Paraclostridium bifermentans
?
-
-
-
-
additional information
substrate specificity
349709
Paraclostridium bifermentans DPH-1
?
-
-
-
-
additional information
broad substrate specificity, degrades various chlorinated aliphatic compounds
349709
Paraclostridium bifermentans DPH-1
?
-
-
-
-
tetrachloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349709
Paraclostridium bifermentans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans DPH-1
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349709
Paraclostridium bifermentans DPH-1
trichloroethene + chloride + acceptor
-
-
-
?
trans-1,2-dichloroethylene + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans DPH-1
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, homodimer, SDS-PAGE; 2 * 35700, homodimer, MALDI-TOF/MS
Paraclostridium bifermentans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Paraclostridium bifermentans
35
-
-
Paraclostridium bifermentans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
35
relatively stable between
Paraclostridium bifermentans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Paraclostridium bifermentans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8
most stable between
Paraclostridium bifermentans
Cofactor
Cofactor
Commentary
Organism
Structure
corrinoid
Co2+ corrinoid
Paraclostridium bifermentans
additional information
no effect by NADH
Paraclostridium bifermentans
Application (protein specific)
Application
Commentary
Organism
agriculture
bioremediation of chloroethylenes, multiple chlorinated aliphatic molecules, in contaminated sites and water, anaerobic reductive dehalogenation of toxic xenobiotic; can play important role in breakdown of perchloroethene and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances
Paraclostridium bifermentans
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Paraclostridium bifermentans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
corrinoid
Co2+ corrinoid
Paraclostridium bifermentans
additional information
no effect by NADH
Paraclostridium bifermentans
General Stability (protein specific)
General Stability
Organism
enzyme generally unstable
Paraclostridium bifermentans
enzyme oxygen sensitive
Paraclostridium bifermentans
purified enzyme not stable, 50% loss of activity at -30°C
Paraclostridium bifermentans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
no effect, no inhibition
Paraclostridium bifermentans
Propyl iodide
1-iodopropane; 90% inhibition by mixture of propyl iodide and titanium citrate in the dark, light restores about 95% of initial activity; light-reversible inactivation in the presence of titanium(III)citrate as electron donor
Paraclostridium bifermentans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
enzyme kinetics and kinetic parameters
Paraclostridium bifermentans
0.012
-
tetrachloroethene
-
Paraclostridium bifermentans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
not an integral membrane protein but a peripheral membrane protein
Paraclostridium bifermentans
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cobalamin
cobalamin-containing enzyme; no significant effect of cyanocobalamin
Paraclostridium bifermentans
Cobalt
Co2+ corrinoid
Paraclostridium bifermentans
additional information
independent of metal ions
Paraclostridium bifermentans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, homodimer, SDS-PAGE
Paraclostridium bifermentans
35700
-
2 * 35700, homodimer, MALDI-TOF/MS
Paraclostridium bifermentans
70000
-
gel filtration
Paraclostridium bifermentans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tetrachloroethene + reduced acceptor
Paraclostridium bifermentans
-
trichloroethene + chloride + acceptor
-
-
?
tetrachloroethene + reduced acceptor
Paraclostridium bifermentans DPH-1
-
trichloroethene + chloride + acceptor
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
-
Paraclostridium bifermentans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Paraclostridium bifermentans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.086
-
-
Paraclostridium bifermentans
Storage Stability (protein specific)
Storage Stability
Organism
-30°C, purified enzyme not stable, 50% loss of activity
Paraclostridium bifermentans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,1,2-trichloroethane + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
1,1-dichloroethylene + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
1,2-dichloropropane + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
cis-1,2-dichloroethylene + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
additional information
substrate specificity
349709
Paraclostridium bifermentans
?
-
-
-
-
additional information
broad substrate specificity, degrades various chlorinated aliphatic compounds
349709
Paraclostridium bifermentans
?
-
-
-
-
additional information
substrate specificity
349709
Paraclostridium bifermentans DPH-1
?
-
-
-
-
additional information
broad substrate specificity, degrades various chlorinated aliphatic compounds
349709
Paraclostridium bifermentans DPH-1
?
-
-
-
-
tetrachloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349709
Paraclostridium bifermentans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans DPH-1
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349709
Paraclostridium bifermentans DPH-1
trichloroethene + chloride + acceptor
-
-
-
?
trans-1,2-dichloroethylene + reduced acceptor
-
349709
Paraclostridium bifermentans
? + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
-
349709
Paraclostridium bifermentans DPH-1
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, homodimer, SDS-PAGE; 2 * 35700, homodimer, MALDI-TOF/MS
Paraclostridium bifermentans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Paraclostridium bifermentans
35
-
-
Paraclostridium bifermentans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
35
relatively stable between
Paraclostridium bifermentans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Paraclostridium bifermentans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8
most stable between
Paraclostridium bifermentans
Other publictions for EC 1.21.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744924
Gadkari
Reductive tetrachloroethene d ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSMZ 12446
FEMS Microbiol. Ecol.
94
0000
2018
-
-
-
-
-
-
-
-
-
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-
2
-
4
-
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-
-
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2
1
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2
-
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744925
Gadkari
Reductive tetrachloroethene d ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
FEMS Microbiol. Ecol.
94
doi: 10.1093/femsec/fix176
2018
-
-
-
-
-
-
-
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2
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9
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2
1
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2
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2
1
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-
744130
Zhao
Microbial reductive dehalogen ...
Dehalobacter sp. THM1
Appl. Microbiol. Biotechnol.
101
5481-5492
2017
-
-
-
-
-
-
-
-
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2
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3
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3
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744468
Ismaeil
Identification of multiple de ...
Dehalococcoides sp. YN3
BioMed Res. Int.
2017
9191086
2017
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
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4
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4
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4
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744884
Kunze
Subtle changes in the active ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
FEBS J.
284
3520-3535
2017
-
-
-
-
8
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
4
1
-
-
-
-
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-
-
-
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-
8
-
-
-
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2
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-
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-
4
1
-
-
-
-
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-
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-
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-
-
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-
744981
Alfan-Guzman
Isolation and characterizatio ...
Dehalobacter sp. TeCB1
Front. Microbiol.
8
558
2017
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
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4
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2
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4
-
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-
-
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-
740462
Zhao
Genomic characterization of De ...
Dehalococcoides mccartyi 11a5, Dehalococcoides mccartyi
FEMS Microbiol. Ecol.
93
fiw235
2016
-
-
-
-
-
-
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-
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4
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2
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-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746490
Matturro
Reductive dechlorination of t ...
Dehalococcoides mccartyi
Sci. Total Environ.
545-546
445-452
2016
-
-
-
-
-
-
-
-
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4
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4
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4
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4
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4
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-
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-
744798
Kranzioch
Chloroethene degradation and ...
Dehalococcoides sp.
Environ. Sci. Pollut. Res. Int.
22
3138-3148
2015
-
-
-
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1
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1
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1
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1
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1
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744800
Wang
Genomic Ccharacterization of ...
Dehalococcoides mccartyi, Dehalococcoides mccartyi JNA
Environ. Sci. Technol.
49
14319-14325
2015
-
-
-
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4
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4
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4
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