BRENDA - Enzyme Database show
show all sequences of 1.21.99.5

Exogenous 5,6-dimethylbenzimidazole caused production of a non-functional tetrachloroethene reductive dehalogenase in Sulfurospirillum multivorans

Keller, S.; Ruetz, M.; Kunze, C.; Kraeutler, B.; Diekert, G.; Schubert, T.; Environ. Microbiol. 16, 3361-3369 (2014)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53300
-
x * 53300, SDS-PAGE, mature protein
Sulfurospirillum multivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfurospirillum multivorans
W6EQP0
-
-
Sulfurospirillum multivorans DSM 12446
W6EQP0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of cofoactor norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
727374
Sulfurospirillum multivorans
?
-
-
-
-
additional information
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of cofoactor norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
727374
Sulfurospirillum multivorans DSM 12446
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 53300, SDS-PAGE, mature protein
Sulfurospirillum multivorans
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
Sulfurospirillum multivorans
vitamin B12
corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion
Sulfurospirillum multivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
Sulfurospirillum multivorans
vitamin B12
corrinoid cofactor is norpseudo-B12, adenine instead of 5,6-dimethylbenzimidazole serves as lower ligand base of the central cobalt ion
Sulfurospirillum multivorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53300
-
x * 53300, SDS-PAGE, mature protein
Sulfurospirillum multivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of cofoactor norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
727374
Sulfurospirillum multivorans
?
-
-
-
-
additional information
anaerobic growth in presence of dimethylbenzimidazole leads to replacement of the adenine moiety in the nucleotide loop of cofoactor norpseudo-B12 by dimethylbenzimidazole. The formation of the dimethylbenzimidazole-containing nor-B12 severely affects tetrachloroethene-dependent growth and the PceA activity. In dimethylbenzimidazole-treated cells processing of the cytoplasmic PceA precursor is impeded. PceA enriched from cells cultivated with dimethylbenzimidazole contains nor-B12. Nor-B12 purified from cells grown in the presence of dimethylbenzimidazole mediates the abiotic reductive dehalogenation of trichloroacetate to dichloroacetate at a 25fold lower rate in comparison with norpseudo-B12
727374
Sulfurospirillum multivorans DSM 12446
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53300, SDS-PAGE, mature protein
Sulfurospirillum multivorans
Other publictions for EC 1.21.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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744468
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2017
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2017
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4
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744884
Kunze
Subtle changes in the active ...
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284
3520-3535
2017
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-
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8
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2
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10
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4
1
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8
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4
1
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744981
Alfan-Guzman
Isolation and characterizatio ...
Dehalobacter sp. TeCB1
Front. Microbiol.
8
558
2017
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2
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4
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4
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740462
Zhao
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93
fiw235
2016
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4
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2
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1
1
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746490
Matturro
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Dehalococcoides mccartyi
Sci. Total Environ.
545-546
445-452
2016
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4
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744798
Kranzioch
Chloroethene degradation and ...
Dehalococcoides sp.
Environ. Sci. Pollut. Res. Int.
22
3138-3148
2015
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744800
Wang
Genomic Ccharacterization of ...
Dehalococcoides mccartyi, Dehalococcoides mccartyi JNA
Environ. Sci. Technol.
49
14319-14325
2015
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4
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727374
Keller
Exogenous 5,6-dimethylbenzimid ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSM 12446
Environ. Microbiol.
16
3361-3369
2014
-
-
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1
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5
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2
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2
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2
1
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739904
Mac Nelly
Functional heterologous produc ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense DSM 10664, Desulfitobacterium hafniense Y51
Appl. Environ. Microbiol.
80
4313-4322
2014
-
-
1
-
1
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8
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14
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2
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2
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14
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740384
Renpenning
Combined C and Cl isotope effe ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSM 12446
Environ. Sci. Technol.
48
11837-11845
2014
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4
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4
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726745
Buttet
Functional genotyping of Sulfu ...
Sulfurospirillum sp. mixed culture SL2
Appl. Environ. Microbiol.
79
6941-6947
2013
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2
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728432
Lohner
Identification of a reductive ...
Shewanella sediminis
Philos. Trans. R. Soc. Lond. B Biol. Sci.
368
20120326
2013
1
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1
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1
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726731
Reinhold
Impact of vitamin B12 on forma ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
Appl. Environ. Microbiol.
78
8025-8032
2012
1
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11
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714522
Kimoto
Cloning of a novel dehalogenas ...
uncultured bacterium
Biosci. Biotechnol. Biochem.
74
1290-1292
2010
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715210
Chow
Identification and transcripti ...
Dehalococcoides sp., Dehalococcoides sp. MB
ISME J.
4
1020-1030
2010
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1
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1
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686429
Schmitz
Evidence for a radical mechani ...
Sulfurospirillum multivorans
Environ. Sci. Technol.
41
7370-7375
2007
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671474
Futagami
Effects of chloromethanes on g ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
Appl. Environ. Microbiol.
72
5998-6003
2006
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3
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8
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Characterization of hydrogenas ...
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3
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1
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1
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12
1
1
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Characterization of the corrin ...
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2
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1
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349704
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13
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7
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13
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1
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A non-dechlorinating strain of ...
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349705
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Two distinct enzyme systems ar ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense TCE1, Desulfitobacterium sp. PCE1
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Purification, cloning, and seq ...
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740468
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Purification and characterizat ...
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Schumacher
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Neumann
Purification and characterizat ...
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