BRENDA - Enzyme Database
show all sequences of 1.21.99.5

Purification and characterization of the tetrachloroethene reductive dehalogenase of strain PCE-S

Miller, E.; Wohlfarth, G.; Diekert, G.; Arch. Microbiol. 169, 497-502 (1998)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
suitable for tetrachloroethene bioremediation
Desulfitobacterium sp. PCE-S
General Stability
General Stability
Organism
oxygen-sensitive with half-life of approximately 50 min
Desulfitobacterium sp. PCE-S
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by ammonium ions
Desulfitobacterium sp. PCE-S
Propyl iodide
1-iodopropane; 1-iodopropane, photoreversible inactivation
Desulfitobacterium sp. PCE-S
trichloroethene
substrate inhibition, higher concentrations of trichloroethene: inhibition of PCE dehalogenase, Ki: 1.3 mM
Desulfitobacterium sp. PCE-S
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
enzyme kinetics and kinetic parameters
Desulfitobacterium sp. PCE-S
0.004
-
trichloroethene
-
Desulfitobacterium sp. PCE-S
0.01
-
tetrachloroethene
-
Desulfitobacterium sp. PCE-S
0.3
-
methyl viologen
at a tetrachloroethene concentration of 1 mM
Desulfitobacterium sp. PCE-S
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
membrane-bound
Desulfitobacterium sp. PCE-S
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cobalamin
dicyano-cob(III)alamin
Desulfitobacterium sp. PCE-S
Cobalt
cobalt content: 1.0 mol of cobalt/mol of protein; contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit
Desulfitobacterium sp. PCE-S
Fe2+
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; two Fe4-S4 clusters; two Fe/S clusters
Desulfitobacterium sp. PCE-S
Iron-sulfur cluster
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; iron-sulfur protein; two Fe4-S4 clusters
Desulfitobacterium sp. PCE-S
iron-sulfur clusters
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; two Fe4-S4 clusters
Desulfitobacterium sp. PCE-S
NH4+
no effect, no activation by ammonium ions
Desulfitobacterium sp. PCE-S
S
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; two Fe4-S4 clusters; two Fe/S clusters
Desulfitobacterium sp. PCE-S
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE
Desulfitobacterium sp. PCE-S
200000
-
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, gel filtration
Desulfitobacterium sp. PCE-S
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetrachloroethene + reduced acceptor
Desulfitobacterium sp. PCE-S
-
trichloroethene + chloride + acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Desulfitobacterium sp. PCE-S
-
strictly anaerobic, gram-positive bacterium
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Desulfitobacterium sp. PCE-S
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
39
-
-
Desulfitobacterium sp. PCE-S
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
substrate specificity
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
additional information
enzyme quite specific
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
additional information
very high substrate specificity
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
additional information
no activity with other chloroethenes or chloromethanes or chlorinated aromatic compounds, no activity with carbon tetrachloride, 1,1-dichloroethene, trans-1,2-dichloroethene, cis-1,2-dichloroethene, 3-chlorobenzoate, 3-chloro-4-hydroxyphenol, pentachlorophenol, 2,4,6-trichlorophenol, 2,4,5-trichlorophenol
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
tetrachloroethene + reduced acceptor
-
349706
Desulfitobacterium sp. PCE-S
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349706
Desulfitobacterium sp. PCE-S
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349706
Desulfitobacterium sp. PCE-S
trichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349706
Desulfitobacterium sp. PCE-S
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
70% of activity compared with tetrachloroethene
349706
Desulfitobacterium sp. PCE-S
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, probably a monomeric 65-kDa protein, SDS-PAGE
Desulfitobacterium sp. PCE-S
More
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo
Desulfitobacterium sp. PCE-S
trimer
3 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE
Desulfitobacterium sp. PCE-S
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
approximately
Desulfitobacterium sp. PCE-S
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
approximately
Desulfitobacterium sp. PCE-S
Cofactor
Cofactor
Commentary
Organism
Structure
corrinoid
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; corrinoid protein; dicyano-cobalamin; dicyano-cob(III)alamin
Desulfitobacterium sp. PCE-S
Application (protein specific)
Application
Commentary
Organism
agriculture
suitable for tetrachloroethene bioremediation
Desulfitobacterium sp. PCE-S
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
corrinoid
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; corrinoid protein; dicyano-cobalamin; dicyano-cob(III)alamin
Desulfitobacterium sp. PCE-S
General Stability (protein specific)
General Stability
Organism
oxygen-sensitive with half-life of approximately 50 min
Desulfitobacterium sp. PCE-S
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by ammonium ions
Desulfitobacterium sp. PCE-S
Propyl iodide
1-iodopropane; 1-iodopropane, photoreversible inactivation
Desulfitobacterium sp. PCE-S
trichloroethene
substrate inhibition, higher concentrations of trichloroethene: inhibition of PCE dehalogenase, Ki: 1.3 mM
Desulfitobacterium sp. PCE-S
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
enzyme kinetics and kinetic parameters
Desulfitobacterium sp. PCE-S
0.004
-
trichloroethene
-
Desulfitobacterium sp. PCE-S
0.01
-
tetrachloroethene
-
Desulfitobacterium sp. PCE-S
0.3
-
methyl viologen
at a tetrachloroethene concentration of 1 mM
Desulfitobacterium sp. PCE-S
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
membrane-bound
Desulfitobacterium sp. PCE-S
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cobalamin
dicyano-cob(III)alamin
Desulfitobacterium sp. PCE-S
Cobalt
cobalt content: 1.0 mol of cobalt/mol of protein; contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit
Desulfitobacterium sp. PCE-S
Fe2+
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; two Fe4-S4 clusters; two Fe/S clusters
Desulfitobacterium sp. PCE-S
Iron-sulfur cluster
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; iron-sulfur protein; two Fe4-S4 clusters
Desulfitobacterium sp. PCE-S
iron-sulfur clusters
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; two Fe4-S4 clusters
Desulfitobacterium sp. PCE-S
NH4+
no effect, no activation by ammonium ions
Desulfitobacterium sp. PCE-S
S
contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit; two Fe4-S4 clusters; two Fe/S clusters
Desulfitobacterium sp. PCE-S
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE
Desulfitobacterium sp. PCE-S
200000
-
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, gel filtration
Desulfitobacterium sp. PCE-S
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetrachloroethene + reduced acceptor
Desulfitobacterium sp. PCE-S
-
trichloroethene + chloride + acceptor
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfitobacterium sp. PCE-S
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
39
-
-
Desulfitobacterium sp. PCE-S
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
substrate specificity
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
additional information
enzyme quite specific
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
additional information
very high substrate specificity
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
additional information
no activity with other chloroethenes or chloromethanes or chlorinated aromatic compounds, no activity with carbon tetrachloride, 1,1-dichloroethene, trans-1,2-dichloroethene, cis-1,2-dichloroethene, 3-chlorobenzoate, 3-chloro-4-hydroxyphenol, pentachlorophenol, 2,4,6-trichlorophenol, 2,4,5-trichlorophenol
349706
Desulfitobacterium sp. PCE-S
?
-
-
-
-
tetrachloroethene + reduced acceptor
-
349706
Desulfitobacterium sp. PCE-S
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349706
Desulfitobacterium sp. PCE-S
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349706
Desulfitobacterium sp. PCE-S
trichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349706
Desulfitobacterium sp. PCE-S
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
70% of activity compared with tetrachloroethene
349706
Desulfitobacterium sp. PCE-S
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, probably a monomeric 65-kDa protein, SDS-PAGE
Desulfitobacterium sp. PCE-S
More
membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo
Desulfitobacterium sp. PCE-S
trimer
3 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE
Desulfitobacterium sp. PCE-S
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
approximately
Desulfitobacterium sp. PCE-S
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
approximately
Desulfitobacterium sp. PCE-S
Other publictions for EC 1.21.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744924
Gadkari
Reductive tetrachloroethene d ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSMZ 12446
FEMS Microbiol. Ecol.
94
0000
2018
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744925
Gadkari
Reductive tetrachloroethene d ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
FEMS Microbiol. Ecol.
94
doi: 10.1093/femsec/fix176
2018
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744130
Zhao
Microbial reductive dehalogen ...
Dehalobacter sp. THM1
Appl. Microbiol. Biotechnol.
101
5481-5492
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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3
-
3
-
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-
-
3
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
744468
Ismaeil
Identification of multiple de ...
Dehalococcoides sp. YN3
BioMed Res. Int.
2017
9191086
2017
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
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4
-
2
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4
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-
-
4
-
-
-
-
-
-
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-
-
-
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-
-
-
-
744884
Kunze
Subtle changes in the active ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
FEBS J.
284
3520-3535
2017
-
-
-
-
8
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
4
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
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-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744981
Alfan-Guzman
Isolation and characterizatio ...
Dehalobacter sp. TeCB1
Front. Microbiol.
8
558
2017
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
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-
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4
-
4
-
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-
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-
2
-
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740462
Zhao
Genomic characterization of De ...
Dehalococcoides mccartyi 11a5, Dehalococcoides mccartyi
FEMS Microbiol. Ecol.
93
fiw235
2016
-
-
-
-
-
-
-
-
-
-
-
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4
-
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2
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746490
Matturro
Reductive dechlorination of t ...
Dehalococcoides mccartyi
Sci. Total Environ.
545-546
445-452
2016
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
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-
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4
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4
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-
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-
744798
Kranzioch
Chloroethene degradation and ...
Dehalococcoides sp.
Environ. Sci. Pollut. Res. Int.
22
3138-3148
2015
-
-
-
-
-
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1
-
1
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1
-
1
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1
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1
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744800
Wang
Genomic Ccharacterization of ...
Dehalococcoides mccartyi, Dehalococcoides mccartyi JNA
Environ. Sci. Technol.
49
14319-14325
2015
-
-
-
-
-
-
-
-
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-
4
-
4
-
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4
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-
-
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-
727374
Keller
Exogenous 5,6-dimethylbenzimid ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSM 12446
Environ. Microbiol.
16
3361-3369
2014
-
-
-
-
-
-
-
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-
-
1
-
-
5
-
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1
-
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-
-
-
-
739904
Mac Nelly
Functional heterologous produc ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense DSM 10664, Desulfitobacterium hafniense Y51
Appl. Environ. Microbiol.
80
4313-4322
2014
-
-
1
-
1
-
-
-
-
-
-
-
-
9
-
-
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14
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-
14
-
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-
-
-
-
-
-
-
-
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-
-
740384
Renpenning
Combined C and Cl isotope effe ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSM 12446
Environ. Sci. Technol.
48
11837-11845
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
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4
-
1
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-
-
4
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
726745
Buttet
Functional genotyping of Sulfu ...
Sulfurospirillum sp. mixed culture SL2
Appl. Environ. Microbiol.
79
6941-6947
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
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-
2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
728432
Lohner
Identification of a reductive ...
Shewanella sediminis
Philos. Trans. R. Soc. Lond. B Biol. Sci.
368
20120326
2013
1
-
-
-
-
-
-
1
-
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-
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3
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-
1
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-
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1
-
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1
-
-
726731
Reinhold
Impact of vitamin B12 on forma ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
Appl. Environ. Microbiol.
78
8025-8032
2012
1
-
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-
-
-
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-
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-
-
13
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-
-
-
-
-
-
-
-
1
1
1
1
-
-
714522
Kimoto
Cloning of a novel dehalogenas ...
uncultured bacterium
Biosci. Biotechnol. Biochem.
74
1290-1292
2010
-
-
-
-
-
-
-
-
-
-
2
1
-
3
-
-
-
-
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-
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-
4
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1
-
-
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1
-
-
-
-
-
-
-
-
-
715210
Chow
Identification and transcripti ...
Dehalococcoides sp., Dehalococcoides sp. MB
ISME J.
4
1020-1030
2010
-
-
-
-
-
-
-
-
-
-
1
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3
-
-
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2
-
-
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-
-
-
-
-
-
1
-
-
1
-
-
686429
Schmitz
Evidence for a radical mechani ...
Sulfurospirillum multivorans
Environ. Sci. Technol.
41
7370-7375
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671474
Futagami
Effects of chloromethanes on g ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
Appl. Environ. Microbiol.
72
5998-6003
2006
-
-
-
-
-
-
3
-
-
-
-
-
-
8
-
-
-
-
-
-
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2
-
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-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
671649
John
Growth substrate dependent loc ...
Sulfurospirillum multivorans
Arch. Microbiol.
186
99-106
2006
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
657605
Nijenhuis
Characterization of hydrogenas ...
Dehalococcoides mccartyi
Appl. Environ. Microbiol.
71
1664-1667
2005
-
-
-
-
-
-
-
-
2
-
-
1
-
3
-
-
-
-
-
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4
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674149
Costentin
Does catalysis of reductive de ...
synthetic construct
J. Am. Chem. Soc.
127
12154-12155
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
674151
Diekert
Electroenzymatic reactions. In ...
Sulfurospirillum multivorans
J. Am. Chem. Soc.
127
13583-13588
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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1
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
675219
Furukawa
Biochemical and molecular char ...
Desulfitobacterium sp., Desulfitobacterium sp. Y51
J. Ind. Microbiol. Biotechnol.
32
534-541
2005
-
-
-
-
-
-
3
6
1
-
1
-
-
5
-
1
-
-
1
-
1
-
12
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-
1
-
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1
-
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-
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1
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-
12
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657587
Maillard
Characterization of the corrin ...
Dehalobacter restrictus, Desulfitobacterium hafniense
Appl. Environ. Microbiol.
69
4628-4638
2003
-
-
2
-
-
-
2
3
2
4
4
2
-
8
1
-
2
-
-
-
1
-
7
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
2
2
-
-
-
-
2
-
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2
4
4
2
-
1
-
2
-
-
1
-
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
349704
Neumann
Tetrachloroethene reductive de ...
Sulfurospirillum multivorans
Arch. Microbiol.
177
420-426
2002
-
1
-
-
-
-
2
7
-
4
-
1
-
5
-
-
-
-
-
-
-
-
13
-
-
1
-
-
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-
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-
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-
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-
-
13
-
1
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1
-
-
-
-
-
-
-
-
-
657689
Siebert
A non-dechlorinating strain of ...
Sulfurospirillum multivorans
Arch. Microbiol.
178
443-449
2002
-
-
-
-
-
-
-
-
-
2
2
1
-
3
-
-
1
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-
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-
-
-
-
349705
Van de Pas
Two distinct enzyme systems ar ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense TCE1, Desulfitobacterium sp. PCE1
Arch. Microbiol.
176
165-169
2001
-
-
-
-
-
-
4
-
2
2
2
3
-
5
-
-
2
-
-
-
2
-
15
2
-
2
-
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-
2
-
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-
-
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-
-
4
-
-
2
2
2
3
-
-
-
2
-
-
2
-
15
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
349709
Okeke
Purification, cloning, and seq ...
Paraclostridium bifermentans, Paraclostridium bifermentans DPH-1
Can. J. Microbiol.
47
448-456
2001
-
1
1
-
-
3
2
2
1
3
3
2
-
3
-
1
1
-
-
-
1
1
15
1
-
2
-
1
-
1
-
1
2
-
-
-
-
1
1
2
-
-
3
-
2
-
2
1
3
3
2
-
-
1
1
-
-
1
1
15
1
2
-
1
-
1
-
1
-
-
-
-
-
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-
740468
Holliger
-
Reductive dechlorination in th ...
uncultured bacterium
FEMS Microbiol. Rev.
22
383-398
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
349703
Magnuson
Reductive dechlorination of te ...
Dehalococcoides mccartyi
Appl. Environ. Microbiol.
64
1270-1275
1998
-
1
-
-
-
3
11
-
1
5
1
1
-
3
-
-
1
1
-
-
1
-
6
1
-
-
-
-
-
-
-
3
2
-
-
-
-
1
-
2
-
-
3
-
11
-
-
1
5
1
1
-
-
-
1
-
-
1
-
6
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
349706
Miller
Purification and characterizat ...
Desulfitobacterium sp. PCE-S
Arch. Microbiol.
169
497-502
1998
-
1
-
-
-
1
3
4
1
7
2
1
-
3
-
-
1
-
-
-
1
-
9
3
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
1
-
3
-
4
1
7
2
1
-
-
-
1
-
-
1
-
9
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
349708
Neumann
Tetrachloroethene dehalogenase ...
Sulfurospirillum multivorans
J. Bacteriol.
180
4140-4145
1998
-
-
1
-
-
-
-
-
2
6
4
1
-
6
-
1
1
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
6
4
1
-
-
1
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349701
Schumacher
Redox chemistry of cobalamin a ...
Dehalobacter restrictus
FEBS Lett.
409
421-425
1997
-
-
-
-
-
-
1
-
1
6
1
3
-
2
-
-
1
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
6
1
3
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740383
Glod
-
Corrinoid-mediated reduction o ...
synthetic construct
Environ. Sci. Technol.
31
253-260
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349700
Neumann
Purification and characterizat ...
Sulfurospirillum multivorans
J. Biol. Chem.
271
16515-16519
1996
-
-
-
-
-
1
10
4
3
8
2
2
-
3
-
-
1
1
-
-
2
-
9
1
-
2
-
1
-
2
-
-
2
-
-
-
-
-
-
2
-
-
1
-
10
-
4
3
8
2
2
-
-
-
1
-
-
2
-
9
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
349702
Schumacher
The proton/electron ratio of t ...
Dehalobacter restrictus
J. Bacteriol.
178
2328-2333
1996
-
1
-
-
-
-
1
1
2
1
-
1
-
2
-
-
-
1
-
-
1
-
4
-
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
1
-
1
2
1
-
1
-
-
-
-
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
349707
Neumann
-
Properties of tetrachloroethen ...
Sulfurospirillum multivorans
Arch. Microbiol.
163
276-281
1995
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1
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7
1
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2
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1
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1
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1
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4
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1
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1
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2
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1
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2
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1
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7
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1
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1
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4
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