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Literature summary for 1.21.99.5 extracted from

  • Miller, E.; Wohlfarth, G.; Diekert, G.
    Purification and characterization of the tetrachloroethene reductive dehalogenase of strain PCE-S (1998), Arch. Microbiol., 169, 497-502.
    View publication on PubMed
Search for more literature for 1.21.99.5

Application

Application Comment Organism
agriculture suitable for tetrachloroethene bioremediation Desulfitobacterium hafniense

General Stability

General Stability Organism
oxygen-sensitive with half-life of approximately 50 min Desulfitobacterium hafniense

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by ammonium ions Desulfitobacterium hafniense
Propyl iodide 1-iodopropane; 1-iodopropane, photoreversible inactivation Desulfitobacterium hafniense
trichloroethene substrate inhibition, higher concentrations of trichloroethene: inhibition of PCE dehalogenase, Ki: 1.3 mM Desulfitobacterium hafniense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme kinetics and kinetic parameters Desulfitobacterium hafniense
0.004
-
 trichloroethene
-
 Desulfitobacterium hafniense
0.01
-
 tetrachloroethene
-
 Desulfitobacterium hafniense
0.3
-
 methyl viologen at a tetrachloroethene concentration of 1 mM Desulfitobacterium hafniense

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane membrane-bound Desulfitobacterium hafniense
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cobalamin dicyano-cob(III)alamin Desulfitobacterium hafniense
Cobalt contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit Desulfitobacterium hafniense
Cobalt cobalt content: 1.0 mol of cobalt/mol of protein Desulfitobacterium hafniense
Fe2+ contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit Desulfitobacterium hafniense
Fe2+ two Fe/S clusters Desulfitobacterium hafniense
Fe2+ two Fe4-S4 clusters Desulfitobacterium hafniense
Iron-sulfur cluster iron-sulfur protein Desulfitobacterium hafniense
Iron-sulfur cluster contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit Desulfitobacterium hafniense
Iron-sulfur cluster two Fe4-S4 clusters Desulfitobacterium hafniense
iron-sulfur clusters contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit Desulfitobacterium hafniense
iron-sulfur clusters two Fe4-S4 clusters Desulfitobacterium hafniense
NH4+ no effect, no activation by ammonium ions Desulfitobacterium hafniense
S contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit Desulfitobacterium hafniense
S two Fe/S clusters Desulfitobacterium hafniense
S two Fe4-S4 clusters Desulfitobacterium hafniense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE Desulfitobacterium hafniense
200000
-
 membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, gel filtration Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetrachloroethene + reduced acceptor Desulfitobacterium hafniense
-
 trichloroethene + chloride + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Desulfitobacterium hafniense
-
 strictly anaerobic, gram-positive bacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfitobacterium hafniense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39
-
-
 Desulfitobacterium hafniense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Desulfitobacterium hafniense ?
-
 ?
additional information enzyme quite specific Desulfitobacterium hafniense ?
-
 ?
additional information very high substrate specificity Desulfitobacterium hafniense ?
-
 ?
additional information no activity with other chloroethenes or chloromethanes or chlorinated aromatic compounds, no activity with carbon tetrachloride, 1,1-dichloroethene, trans-1,2-dichloroethene, cis-1,2-dichloroethene, 3-chlorobenzoate, 3-chloro-4-hydroxyphenol, pentachlorophenol, 2,4,6-trichlorophenol, 2,4,5-trichlorophenol Desulfitobacterium hafniense ?
-
 ?
tetrachloroethene + reduced acceptor
-
 Desulfitobacterium hafniense trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor artificial electron donor: reduced methyl viologen, physiological electron donor is not known Desulfitobacterium hafniense trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Desulfitobacterium hafniense trichloroethene + chloride + acceptor
-
 ?
trichloroethene + chloride + methyl viologen
-
 Desulfitobacterium hafniense tetrachloroethene + reduced methyl viologen
-
 r
trichloroethene + reduced acceptor artificial electron donor: reduced methyl viologen, physiological electron donor is not known Desulfitobacterium hafniense cis-1,2-dichloroethene + chloride + acceptor
-
 ?
trichloroethene + reduced acceptor 70% of activity compared with tetrachloroethene Desulfitobacterium hafniense cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW of 65000 Da in vivo, probably a monomeric 65-kDa protein, SDS-PAGE Desulfitobacterium hafniense
More membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo Desulfitobacterium hafniense
trimer 3 * 65000, membrane proteins tend to form aggregates due to their high hydrophobicity, apparent MW of 200 kDa for the native enzyme may be a purification artifact, and the enzyme may be essentially monomeric with MW: 65000 Da in vivo, SDS-PAGE Desulfitobacterium hafniense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 approximately Desulfitobacterium hafniense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 approximately Desulfitobacterium hafniense

Cofactor

Cofactor Comment Organism Structure
corrinoid corrinoid protein Desulfitobacterium hafniense
corrinoid dicyano-cobalamin Desulfitobacterium hafniense
corrinoid dicyano-cob(III)alamin Desulfitobacterium hafniense
corrinoid contains 0.7 mol corrinoid, 1.0 mol cobalt, 7.8 mol iron, and 10.3 mol acid-labile sulfur per mol subunit Desulfitobacterium hafniense