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Literature summary for 1.21.99.5 extracted from

  • Maillard, J.; Schumacher, W.; Vazquez, F.; Regeard, C.; Hagen, W.R.; Holliger, C.
    Characterization of the corrinoid iron-sulfur protein tetrachloroethene reductive dehalogenase of Dehalobacter restrictus (2003), Appl. Environ. Microbiol., 69, 4628-4638.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha Dehalobacter restrictus
expressed in Escherichia coli DH5alpha Desulfitobacterium hafniense

Inhibitors

Inhibitors Comment Organism Structure
1-Iodopropane
-
Dehalobacter restrictus
1-Iodopropane
-
Desulfitobacterium hafniense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
tetrachloroethene 30°C Dehalobacter restrictus
0.024
-
reduced methyl viologen 30°C Dehalobacter restrictus
0.047
-
trichloroethene 30°C Dehalobacter restrictus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Dehalobacter restrictus 16020
-
membrane
-
Desulfitobacterium hafniense 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron two [4Fe-4S] cluster Dehalobacter restrictus
Iron two [4Fe-4S] cluster Desulfitobacterium hafniense
sulfur two [4Fe-4S] cluster Dehalobacter restrictus
sulfur two [4Fe-4S] cluster Desulfitobacterium hafniense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
SDS-PAGE Desulfitobacterium hafniense
59430
-
enzyme mass + cobalamin cofactor + iron-sulfur clusters, calculated from deduced amino acid sequence Dehalobacter restrictus
60000
-
SDS-PAGE Dehalobacter restrictus
71000
-
gel filtration Dehalobacter restrictus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetrachloroethene + 2 reduced acceptor Dehalobacter restrictus terminal component of the respiratory chain cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+
-
?
tetrachloroethene + 2 reduced acceptor Desulfitobacterium hafniense terminal component of the respiratory chain cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Dehalobacter restrictus Q8GJ27 DSMZ 9455T
-
Desulfitobacterium hafniense Q8GJ31 strain TCE1, DSMZ 12704, formerly Desulfitobacterium frappieri strain TCE1
-

Oxidation Stability

Oxidation Stability Organism
sensitive to oxygen exposure, half-life of 280 h upon exposure to air Dehalobacter restrictus

Purification (Commentary)

Purification (Comment) Organism
-
Dehalobacter restrictus
-
Desulfitobacterium hafniense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.6
-
purified enzyme, 30°C Dehalobacter restrictus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information trichlofluoroethene, tetrachloromethane, hexachloroethane, tetrachloroethane, trichloroethane, and 1,1,1-trichloro-2,2,2-trifluoroethane cause an enzyme dependent oxidation of reduced methyl viologen Dehalobacter restrictus ?
-
?
tetrachloroethene + 2 reduced acceptor terminal component of the respiratory chain Dehalobacter restrictus cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+
-
?
tetrachloroethene + 2 reduced acceptor terminal component of the respiratory chain Desulfitobacterium hafniense cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+
-
?
tetrachloroethene + reduced methyl viologen via trichloroethene Dehalobacter restrictus cis-1,2-dichloroethene + 2 chloride + methyl viologen
-
?
tetrachloroethene + reduced methyl viologen via trichloroethene Desulfitobacterium hafniense cis-1,2-dichloroethene + 2 chloride + methyl viologen
-
?
trichloroethene + chloride + methyl viologen
-
Dehalobacter restrictus tetrachloroethene + reduced methyl viologen
-
?
trichloroethene + chloride + methyl viologen
-
Desulfitobacterium hafniense tetrachloroethene + reduced methyl viologen
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE, native mass of 71000 determined by gel filtration Dehalobacter restrictus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
Dehalobacter restrictus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Dehalobacter restrictus

Cofactor

Cofactor Comment Organism Structure
Cobalamin
-
Dehalobacter restrictus
corrinoid
-
Desulfitobacterium hafniense