Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha | Dehalobacter restrictus |
expressed in Escherichia coli DH5alpha | Desulfitobacterium hafniense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Iodopropane | - |
Dehalobacter restrictus | |
1-Iodopropane | - |
Desulfitobacterium hafniense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
tetrachloroethene | 30°C | Dehalobacter restrictus | |
0.024 | - |
reduced methyl viologen | 30°C | Dehalobacter restrictus | |
0.047 | - |
trichloroethene | 30°C | Dehalobacter restrictus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Dehalobacter restrictus | 16020 | - |
membrane | - |
Desulfitobacterium hafniense | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | two [4Fe-4S] cluster | Dehalobacter restrictus | |
Iron | two [4Fe-4S] cluster | Desulfitobacterium hafniense | |
sulfur | two [4Fe-4S] cluster | Dehalobacter restrictus | |
sulfur | two [4Fe-4S] cluster | Desulfitobacterium hafniense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
SDS-PAGE | Desulfitobacterium hafniense |
59430 | - |
enzyme mass + cobalamin cofactor + iron-sulfur clusters, calculated from deduced amino acid sequence | Dehalobacter restrictus |
60000 | - |
SDS-PAGE | Dehalobacter restrictus |
71000 | - |
gel filtration | Dehalobacter restrictus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrachloroethene + 2 reduced acceptor | Dehalobacter restrictus | terminal component of the respiratory chain | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
tetrachloroethene + 2 reduced acceptor | Desulfitobacterium hafniense | terminal component of the respiratory chain | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dehalobacter restrictus | Q8GJ27 | DSMZ 9455T | - |
Desulfitobacterium hafniense | Q8GJ31 | strain TCE1, DSMZ 12704, formerly Desulfitobacterium frappieri strain TCE1 | - |
Oxidation Stability | Organism |
---|---|
sensitive to oxygen exposure, half-life of 280 h upon exposure to air | Dehalobacter restrictus |
Purification (Comment) | Organism |
---|---|
- |
Dehalobacter restrictus |
- |
Desulfitobacterium hafniense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.6 | - |
purified enzyme, 30°C | Dehalobacter restrictus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | trichlofluoroethene, tetrachloromethane, hexachloroethane, tetrachloroethane, trichloroethane, and 1,1,1-trichloro-2,2,2-trifluoroethane cause an enzyme dependent oxidation of reduced methyl viologen | Dehalobacter restrictus | ? | - |
? | |
tetrachloroethene + 2 reduced acceptor | terminal component of the respiratory chain | Dehalobacter restrictus | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
tetrachloroethene + 2 reduced acceptor | terminal component of the respiratory chain | Desulfitobacterium hafniense | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
tetrachloroethene + reduced methyl viologen | via trichloroethene | Dehalobacter restrictus | cis-1,2-dichloroethene + 2 chloride + methyl viologen | - |
? | |
tetrachloroethene + reduced methyl viologen | via trichloroethene | Desulfitobacterium hafniense | cis-1,2-dichloroethene + 2 chloride + methyl viologen | - |
? | |
trichloroethene + chloride + methyl viologen | - |
Dehalobacter restrictus | tetrachloroethene + reduced methyl viologen | - |
? | |
trichloroethene + chloride + methyl viologen | - |
Desulfitobacterium hafniense | tetrachloroethene + reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE, native mass of 71000 determined by gel filtration | Dehalobacter restrictus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
- |
Dehalobacter restrictus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Dehalobacter restrictus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | - |
Dehalobacter restrictus | |
corrinoid | - |
Desulfitobacterium hafniense |