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Literature summary for 1.21.99.5 extracted from
- Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway (1998), Appl. Environ. Microbiol., 64, 1270-1275.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | degradation of lipophilic solvent tetrachloroethene, one of the most abundant halogenated xenobiotic pollutants in the environment | Dehalococcoides mccartyi |
General Stability
| General Stability | Organism |
|---|---|
| cysteine stabilizes | Dehalococcoides mccartyi |
| enzyme very labile during purification | Dehalococcoides mccartyi |
| ferrous ammonium sulfate stabilizes | Dehalococcoides mccartyi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Dehalococcoides mccartyi |  |
| CuCl2 | 1 mM cuprous chloride: slight inhibition | Dehalococcoides mccartyi | |
| cyanide | 20 mM sodium cyanide: 20% inhibition | Dehalococcoides mccartyi | |
| Dithionite | 2 mM sodium dithionite: complete inhibition | Dehalococcoides mccartyi | |
| EDTA | no inhibition by 5 mM EDTA | Dehalococcoides mccartyi | |
| Iodoethane | in the presence of titanium(III)citrate: 85% inhibition; light-reversible inhibition | Dehalococcoides mccartyi | |
| additional information | no inhibition by 2 mM sodium sulfate, sodium selenate, sodium sulfide, or 100% carbon monoxide; no inhibition by 5 mM EDTA, bathophenanthroline disulfonate, and 2,2-dipyridyl | Dehalococcoides mccartyi | |
| Propyl iodide | no inhibition in the presence of 1-iodopropane and titanium(III)citrate | Dehalococcoides mccartyi | |
| sulfite | 2 mM sodium sulfite: complete inhibition | Dehalococcoides mccartyi | |
| Zn2+ | - |
Dehalococcoides mccartyi | |
| ZnCl2 | 5 mM zinc cloride: slight inhibition | Dehalococcoides mccartyi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | membrane-bound | Dehalococcoides mccartyi | - |
- |
| cytoplasmic membrane | cell wall-membrane | Dehalococcoides mccartyi | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cobalt | Co(I) corrinoid cofactor | Dehalococcoides mccartyi | |
| Fe2+ | iron-sulfur cluster | Dehalococcoides mccartyi | |
| Iron-sulfur cluster | - |
Dehalococcoides mccartyi | |
| iron-sulfur clusters | - |
Dehalococcoides mccartyi | |
| S | iron-sulfur cluster | Dehalococcoides mccartyi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
PAGE | Dehalococcoides mccartyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | Dehalococcoides mccartyi | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dehalococcoides mccartyi | - |
195 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Dehalococcoides mccartyi |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | mechanism | Dehalococcoides mccartyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.5 | - |
- |
Dehalococcoides mccartyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity | Dehalococcoides mccartyi | ? | - |
? | |
| additional information | no activity with trichloroethene, TCE, cis-dichloroethene, cis-DCE, or vinyl chloride | Dehalococcoides mccartyi | ? | - |
? | |
| additional information | enzyme quite specific | Dehalococcoides mccartyi | ? | - |
? | |
| tetrachloroethene + reduced acceptor | - |
Dehalococcoides mccartyi | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | quite specific for tetrachloroethene | Dehalococcoides mccartyi | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Dehalococcoides mccartyi | trichloroethene + chloride + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, SDS-PAGE | Dehalococcoides mccartyi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable at neutral pH | Dehalococcoides mccartyi |
| 5 | 8 | unstable above pH 8 or below pH 5 | Dehalococcoides mccartyi |
| 7 | - |
stable at neutral pH | Dehalococcoides mccartyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid | Co(I) corrinoid cofactor | Dehalococcoides mccartyi | |
| vitamin B12 | - |
Dehalococcoides mccartyi | |
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