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7.6.2.9: ABC-type quaternary amine transporter

This is an abbreviated version!
For detailed information about ABC-type quaternary amine transporter, go to the full flat file.

Word Map on EC 7.6.2.9

Reaction

ATP
+
H2O
 +
quaternary amine-[quaternary amine-binding protein][side 1]
=
ADP
 +
phosphate
 +
quaternary amine[side 2]
 +
[quaternary amine-binding protein][side 1]

Synonyms

ABC transporter, BetTA.halophytica, EC 3.6.3.32, glycine betaine porter II, glycine betaine transport system permease protein, glycine betaine transporter membrane protein, glycine betaine-binding protein, glycine betaine-binding protein OpuAC, glycine betaine/carnitine/choline-binding protein, glycine/betaine ABC transporter, N288_21505, N288_21525, N288_25665, OpuA, OpuAB, OpuAC, OpuB, OpuBC, OpuC, OpuCC, OpuD, OpuF, OpuF transporter, proline/betaine ABC transporter permease, ProU, proW, Quaternary-amine-transporting ATPase

ECTree

     7 Translocases
         7.6 Catalysing the translocation of other compounds
             7.6.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.6.2.9 ABC-type quaternary amine transporter

Cloned

Cloned on EC 7.6.2.9 - ABC-type quaternary amine transporter

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into pApBetT, transfered to Escherichia coli and then to MKH13 cells, overexpression in freshwater Synechococcus sp. strain PCC7942 cells
cloning of the regulatory region of the glycine betaine transport system
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli, cysteine mutants generated and inserted into pBAD33
expressed in Escherichia coli, DH5-alpha used as host for cloning, MG1655 used as host for mutagenesis
expression in Escherichia coli
expression in Lactococcus lactis
-
expression of OpuAA protein in Escherichia coli
-
gene opuAB, sequence comparisons
gene opuAC, sequence comparisons
gene opuF, functional expression and resonstitution of the OpuF transporter in Bacillus subtilis
genes proW and proX are located on chromosome 2 (Vibrio anguillarum strain MVM425 genome is composed of two circular chromosomes) and encode ABC transporter permease and choline ABC transporter substrate-binding protein (glycine/betaine ABC transporter substrate-binding protein), respectively, DNA and amino acid sequence determination and analyis, sequence comparisons, quantitative real-time RT-PCR expression analysis
A0A077T980; A0A077T7U5; A0A077T7Z1, A0A097A5F2; A0A077T7R8
genes proW, proX, and proV are located on chromosome 2 (Vibrio anguillarum strain MVM425 genome is composed of two circular chromosomes) and encode glycine betaine transporter membrane protein (proline/betaine ABC transporter permease), glycine betaine ABC transporter substrate-binding protein, and glycine betaine/L-proline ABC transporter ATP-binding protein, respectively, DNA and amino acid sequence determination and analyis, sequence comparisons, quantitative real-time RT-PCR expression analysis
A0A077T980; A0A077T7U5; A0A077T7Z1, A0A097A5F2; A0A077T7R8
into the pTOPO vector
-
nucleotide-binding protein domain cloned as an N- or C-terminal His-tagged fusion protein and overexpressed in Escherichia coli BL21 (DE3) under the control of an arabinose-inducible promoter, overexpression of the transmembrane domain only possible in Walker strains BL21(DE3)C41 and C43 under the control of an ITPG- or arabinose-inducible promoter as N- and C-terminal His- and Strep-tagged versions
-
opuA operon, the coding region of the opuA operon and the adjacent opuAR regulatory gene are cloned into the vector pX resulting in plasmid pCA-opuARA, sequence comparisons, recombinant expression in Bacillus subtilis strain TMB118, in which only the proline-specific osmostress OpuE transporter is present, the resulting strain is CAB2. opuAA0 Bacillus infantis-treA reporter gene fusions are constructed using primers opuAR treA Frag1/2 rev and either opuAR treA Frag1/4. Recombinant overexpression of StrepII-tagged OpuAR repressor and mutant derivatives from the P-tet promoter in the Escherichia coli B strain BL21
U5LFB4; U5LHK3; U5LHN4
substrate-binding domain into vector pBKB76, expression in Escherichia coli BL21 (DE3)
-
transmembrane subunits cloned into vector pBAD33 or pET21a, expression in Escherichia coli
-