Literature summary for 7.6.2.9 extracted from

Ma, Y.; Wang, Q.; Gao, X.; Zhang, Y.
Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum (2017), J. Microbiol., 55, 44-55 .

Cloned(Commentary)

Cloned (Comment)	Organism
genes proW and proX are located on chromosome 2 (Vibrio anguillarum strain MVM425 genome is composed of two circular chromosomes) and encode ABC transporter permease and choline ABC transporter substrate-binding protein (glycine/betaine ABC transporter substrate-binding protein), respectively, DNA and amino acid sequence determination and analyis, sequence comparisons, quantitative real-time RT-PCR expression analysis	Vibrio anguillarum serotype O1
genes proW, proX, and proV are located on chromosome 2 (Vibrio anguillarum strain MVM425 genome is composed of two circular chromosomes) and encode glycine betaine transporter membrane protein (proline/betaine ABC transporter permease), glycine betaine ABC transporter substrate-binding protein, and glycine betaine/L-proline ABC transporter ATP-binding protein, respectively, DNA and amino acid sequence determination and analyis, sequence comparisons, quantitative real-time RT-PCR expression analysis	Vibrio anguillarum serotype O1

Cloned (Comment)

Organism

genes proW and proX are located on chromosome 2 (Vibrio anguillarum strain MVM425 genome is composed of two circular chromosomes) and encode ABC transporter permease and choline ABC transporter substrate-binding protein (glycine/betaine ABC transporter substrate-binding protein), respectively, DNA and amino acid sequence determination and analyis, sequence comparisons, quantitative real-time RT-PCR expression analysis

Vibrio anguillarum serotype O1

genes proW, proX, and proV are located on chromosome 2 (Vibrio anguillarum strain MVM425 genome is composed of two circular chromosomes) and encode glycine betaine transporter membrane protein (proline/betaine ABC transporter permease), glycine betaine ABC transporter substrate-binding protein, and glycine betaine/L-proline ABC transporter ATP-binding protein, respectively, DNA and amino acid sequence determination and analyis, sequence comparisons, quantitative real-time RT-PCR expression analysis

Vibrio anguillarum serotype O1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Vibrio anguillarum serotype O1	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Vibrio anguillarum serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1]	Vibrio anguillarum serotype O1	-	ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1]	-	?
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1]	Vibrio anguillarum serotype O1 MVM425	-	ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1]	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio anguillarum serotype O1	A0A077T980 AND A0A077T7U5 AND A0A077T7Z1	genes proW, proX, and proV encoding glycine betaine transporter membrane protein (proline/betaine ABC transporter permease), glycine betaine ABC transporter substrate-binding protein, and glycine betaine/L-proline ABC transporter ATP-binding protein, respectively; isolated from diseased fish suffered from epidemic vibriosis in the fishery in the Northern China Sea	-
Vibrio anguillarum serotype O1	A0A097A5F2 AND A0A077T7R8	genes proW and proX encoding ABC transporter permease and choline ABC transporter substrate-binding protein (glycine/betaine ABC transporter substrate-binding protein), respectively; isolated from diseased fish suffered from epidemic vibriosis in the fishery in the Northern China Sea	-
Vibrio anguillarum serotype O1 MVM425	A0A077T980 AND A0A077T7U5 AND A0A077T7Z1	genes proW, proX, and proV encoding glycine betaine transporter membrane protein (proline/betaine ABC transporter permease), glycine betaine ABC transporter substrate-binding protein, and glycine betaine/L-proline ABC transporter ATP-binding protein, respectively; isolated from diseased fish suffered from epidemic vibriosis in the fishery in the Northern China Sea	-
Vibrio anguillarum serotype O1 MVM425	A0A097A5F2 AND A0A077T7R8	genes proW and proX encoding ABC transporter permease and choline ABC transporter substrate-binding protein (glycine/betaine ABC transporter substrate-binding protein), respectively; isolated from diseased fish suffered from epidemic vibriosis in the fishery in the Northern China Sea	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1]	-	Vibrio anguillarum serotype O1	ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1]	-	?
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1]	-	Vibrio anguillarum serotype O1 MVM425	ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1]	-	?

Synonyms

Synonyms	Comment	Organism
glycine betaine transporter membrane protein	UniProt	Vibrio anguillarum serotype O1
proline/betaine ABC transporter permease	UniProt	Vibrio anguillarum serotype O1
proW	-	Vibrio anguillarum serotype O1
proW	UniProt	Vibrio anguillarum serotype O1

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Vibrio anguillarum serotype O1

General Information

General Information	Comment	Organism
malfunction	in-frame deletion mutation in the two putative ABC transporters and three putative BCCT family transporters associated with glycine betaine uptake cannot block cellular accumulation of betaine glycine in Vibrio anguillarum under cold stress, suggesting the redundant feature in Vibrio anguillarum betaine transporter system	Vibrio anguillarum serotype O1
physiological function	Vibrio anguillarum possesses a putative glycine betaine synthesis system, which is encoded by betABI and synthesizes glycine betaine from its precursor choline. Significant up-regulation of the bet gene at the transcriptional level is noted in log phase in response to cold-stress. The accumulation of betaine glycine is only appearing at low growth temperatures, suggesting that response regulation of both synthesis system and transporter system are cold-dependent. Two putative ABC transporters and three putative BCCT family transporters are associated with glycine betaine uptake. Glycine betaine serves as an effective cold stress protectant, thus the enzyme complex is involved in cold adaptation of Vibrio anguillarum via regulation of the glycine betaine level	Vibrio anguillarum serotype O1
physiological function	Vibrio anguillarum possesses a putative glycine betaine synthesis system, which is encoded by betABI and synthesizes glycine betaine from its precursor choline. Significant upregulation of the bet gene at the transcriptional level is noted in log phase in response to cold-stress. The accumulation of betaine glycine is only appearing at low growth temperatures, suggesting that response regulation of both synthesis system and transporter system are cold-dependent. Two putative ABC transporters and three putative BCCT family transporters are associated with glycine betaine uptake. Glycine betaine serves as an effective cold stress protectant, thus the enzyme complex is involved in cold adaptation of Vibrio anguillarum via regulation of the glycine betaine level	Vibrio anguillarum serotype O1