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Literature summary for 7.6.2.9 extracted from

  • Obis, D.; Guillot, A.; Gripon, J.C.; Renault, P.; Bolotin, A.; Mistou, M.Y.
    Genetic and biochemical characterization of a high-affinity betaine uptake system (BusA) in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters (1999), J. Bacteriol., 181, 6238-6246.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 betaine
-
 Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45679
-
 x * 45679, BusAA + x * 61966, BusAB + x * ?, BusAC, calculation from nucleotide sequence Lactococcus lactis
61966
-
 x * 45679, BusAA + x * 61966, BusAB + x * ?, BusAC, calculation from nucleotide sequence Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + glycine betaine/out Lactococcus lactis enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium ADP + phosphate + glycine betaine/in
-
 ?
ATP + H2O + glycine betaine/out Lactococcus lactis OpuA enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium ADP + phosphate + glycine betaine/in
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 OpuA
-
Lactococcus lactis OpuA
-
 OpuA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + betaine/out
-
 Lactococcus lactis ADP + phosphate + betaine/in
-
 ?
ATP + H2O + betaine/out
-
 Lactococcus lactis OpuA ADP + phosphate + betaine/in
-
 ?
ATP + H2O + glycine betaine/out
-
 Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
ATP + H2O + glycine betaine/out enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
ATP + H2O + glycine betaine/out
-
 Lactococcus lactis OpuA ADP + phosphate + glycine betaine/in
-
 ?
ATP + H2O + glycine betaine/out enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium Lactococcus lactis OpuA ADP + phosphate + glycine betaine/in
-
 ?

Subunits

Subunits Comment Organism
? x * 45679, BusAA + x * 61966, BusAB + x * ?, BusAC, calculation from nucleotide sequence Lactococcus lactis