Literature summary for 7.6.2.9 extracted from

Du, Y.; Shi, W.W.; He, Y.X.; Yang, Y.H.; Zhou, C.Z.; Chen, Y.
Structures of the substrate-binding protein provide insights into the multiple compatible solute binding specificities of the Bacillus subtilis ABC transporter OpuC (2011), Biochem. J., 436, 283-289.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
substrate-binding protein OpuCC in the apo-form and in complex with carnitine, glycine betaine, choline and ectoine to 2.3, 2.7, 2.4, 1.9 and 2.1 A resolution, respectively. OpuCC is composed of two alpha/beta/alpha globular sandwich domains linked by two hinge regions, with a substrate-binding pocket located at the interdomain cleft. Upon substrate binding, the two domains shift towards each other to trap the substrate	Bacillus subtilis

Crystallization (Comment)

Organism

substrate-binding protein OpuCC in the apo-form and in complex with carnitine, glycine betaine, choline and ectoine to 2.3, 2.7, 2.4, 1.9 and 2.1 A resolution, respectively. OpuCC is composed of two alpha/beta/alpha globular sandwich domains linked by two hinge regions, with a substrate-binding pocket located at the interdomain cleft. Upon substrate binding, the two domains shift towards each other to trap the substrate

Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
T94D	shares a quite similar pattern of fluorescence spectrum to that of the paralogue OpuBC. Only choline can trigger obvious changes of fluorescence intensity of mutant T94D, whereas carnitine, GB and ectoine cannot	Bacillus subtilis
Y91A	complete loss of binding affinity	Bacillus subtilis
Y91F	slight decrease in binding affinity	Bacillus subtilis
Y91W	slight decrease in binding affinity	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O32243	-	-

Synonyms

Synonyms	Comment	Organism
glycine betaine/carnitine/choline-binding protein	-	Bacillus subtilis
OpuCC	-	Bacillus subtilis

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Release 2023.1 (January 2023)