6.1.1.17: glutamate-tRNA ligase

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For detailed information about glutamate-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.17

6.1.1.17

synthetases

aminoacyl-trna

aminoacylation

glnrs

trnagln

ampa

ionotropic

excitatory

anticodon

metabotropic

kainate

glu-trnagln

amidotransferase

n-methyl-d-aspartate

misacylated

glutamatergic

mischarging

trna-dependent

glutaminylation

non-nmda

noncognate

nmdars

transamidation

aspartyl-trna

gatcab

aarss

metrs

trnatyr

isoacceptors

lysyl-trna

non-discriminating

misaminoacylation

alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate

ampa-type

glutamate-1-semialdehyde

arginyl-trna

asparaginyl-trna

anticodon-binding

glur2/3

methionyl-trna

trna-binding

drug development

tryptophanyl-trna

Reaction

Synonyms

bifunctional aminoacyl-tRNA synthetase, bifunctional glutamate/proline-tRNA ligase, D-GluRS, discriminating GluRS, EC 2.7.2.13, Ec-GluRS, EPRS, ERS, Ers2, GltX, GluRS, GluRS1, GluRS2, GluRSAt, Glutamate--tRNA ligase, Glutamate-tRNA synthetase, Glutamic acid translase, Glutamic acid tRNA ligase, Glutaminyl-tRNA synthetase, Glutamyl tRNA synthetase, glutamyl-prolyl tRNA synthetase, glutamyl-prolyl-tRNA synthetase, glutamyl-Q tRNAASp synthetase, Glutamyl-transfer ribonucleate synthetase, Glutamyl-transfer ribonucleic acid synthetase, Glutamyl-transfer RNA synthetase, Glutamyl-tRNA synthetase, glutamyl-tRNAsynthetase, GtS, More, P85, surface-exposed glutamyl tRNA synthetase, TM1351, tRNA modifying enzyme, YadB

ECTree

6 Ligases

6.1 Forming carbon-oxygen bonds

6.1.1 Ligases forming aminoacyl-tRNA and related compounds

6.1.1.17 glutamate-tRNA ligase

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Results	in table
10	Activating Compound
54930	AA Sequence
2	Application
1	CAS Registry Number
38	Cloned(Commentary)
43	Cofactor
10	Crystallization (Commentary)
143	Disease/ Diagnostics
6	Expression
22	General Information
3	General Stability
2	IC50 Value
50	Inhibitors
14	kcat/KM [mM/s]
28	Ki Value [mM]
72	KM Value [mM]
20	Localization
43	Metals/Ions
33	Molecular Weight [Da]
61	Natural Substrates/ Products (Substrates)
589	Organism
9	Pathway
101	PDB ID
24	pH Optimum
2	pH Range
5	Posttranslational Modification
27	Protein Variants
32	Purification (Commentary)
11	Reaction
46	Reaction Type
121	Reference
20	Source Tissue
17	Specific Activity [micromol/min/mg]
3	Storage Stability
187	Substrates and Products (Substrate)
38	Subunits
140	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
3	Temperature Range [°C]
8	Temperature Stability [°C]
32	Turnover Number [1/s]

Purification

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Purification on EC 6.1.1.17 - glutamate-tRNA ligase

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10 entries

50fold, recombinant enzyme from overexpression in Bacillus subtilis, to homogeneity

Bacillus subtilis

492295

Ni-NTA column chromatography and gel filtration

Plasmodium falciparum

727963

partial, recombinant enzyme from Escherichia coli with or without N-terminal His-tag

Chlamydia trachomatis

440092

recombinant enzyme

Mycobacterium tuberculosis

P9WFV9

703636

recombinant enzyme to over 98% homogeneity

Pseudomonas aeruginosa

Q9XCL6

745395

recombinant GST-GluRS1 by glutathione affinity chromatography, followed by removal of GST

Acidithiobacillus ferrooxidans

714031

recombinant His-tagged chimeric mutant enzyme or catalytic domain of GluRS from strain BL21(DE3) or the temperature sensitive strain JP1449(DE3) by nickel affinity chromatography

Escherichia coli

690792

recombinant His-tagged ERS from strain BL21(DE3) by nickel affinity chromatography

Escherichia coli

677107

recombinant His-tagged GluRS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Saccharomyces cerevisiae

P46655

676213

recombinant His6-tagged GluRS-N from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration

Saccharomyces cerevisiae

P46655

671048

recombinant N-terminally His6-SUMO2-Gly-tagged K236E/E328A mutant enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and dialysis, tag cleavage by SENP2 protease and another step of nickel affinity chromatography to remove the tag, to over 95% purity, followed by ultrafiltration

Escherichia coli

743955

recombinant tagged wild-type and mutant enzymes from Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL by affinity chromatography and gel filtration

2 entries

recombinant TM1351 from Escherichia coli strain Rosetta2 (DE3) by anion exchange chromatography and gel filtration

Thermotoga maritima

713616

six-h procedure

Escherichia coli

202