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6.1.1.17: glutamate-tRNA ligase

This is an abbreviated version!
For detailed information about glutamate-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.17

Reaction

ATP
+
L-glutamate
 +
tRNAGlu
=
AMP
 +
diphosphate
 +
L-glutamyl-tRNAGlu

Synonyms

bifunctional aminoacyl-tRNA synthetase, bifunctional glutamate/proline-tRNA ligase, D-GluRS, discriminating GluRS, EC 2.7.2.13, Ec-GluRS, EPRS, ERS, Ers2, GltX, GluRS, GluRS1, GluRS2, GluRSAt, Glutamate--tRNA ligase, Glutamate-tRNA synthetase, Glutamic acid translase, Glutamic acid tRNA ligase, Glutaminyl-tRNA synthetase, Glutamyl tRNA synthetase, glutamyl-prolyl tRNA synthetase, glutamyl-prolyl-tRNA synthetase, glutamyl-Q tRNAASp synthetase, Glutamyl-transfer ribonucleate synthetase, Glutamyl-transfer ribonucleic acid synthetase, Glutamyl-transfer RNA synthetase, Glutamyl-tRNA synthetase, glutamyl-tRNAsynthetase, GtS, More, P85, surface-exposed glutamyl tRNA synthetase, TM1351, tRNA modifying enzyme, YadB

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.17 glutamate-tRNA ligase

Cloned

Cloned on EC 6.1.1.17 - glutamate-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
DNA and amino acid sequence determination and analysis, full length gene, single gene, expression in Escherichia coli BL21 as S-tagged enzyme
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli KRX cells
-
expression in Escherichia coli
expression of C-terminally His-tagged, full-length EPRS
-
expression of His-tagged ERS in strain BL21(DE3)
-
expression of His6-tagged GluRS-N, comprising residues 1-197, 17-207 and 1-207, in Escherichia coli strain BL21(DE3)
expression of the His-tagged chimeric mutant enzyme and of the catalytic domain of GluRS in Escherichia coli strain BL21(DE3) or the temperature sensitive strain JP1449(DE3)
-
expression of wild-type and mutant His-tagged GluRSs in Escherichia coli strain BL21(DE3)
gene EPRS, quantitative PCR -based expression //analysis
-
gene gltX is encoded in an operon comprising the genes gltX, cysE, and cysS, after transcription the polycistronic mRNA is cleaved into the gltX transcript and the cysE-cysS transcript due to an internal signal structure and probably by self-cleavage, analysis, the gltX mRNA may be stabilized by secondary structures at the 3'-end and the 5'-end, expression in Escherichia coli and in vitro transcription
-
gene gltX mutant, mutant enzyme expression in Escherichia coli temperature-sensitive mutant strain
-
gene gltX, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
gene gltX, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme expression
gene gltX, expression in Escherichia coli is lethal, but a modulated enzyme, lacking a part of the tRNA acceptor stem binding domain, can be recombinantly expressed in Escherichia coli DH5alpha, overexpression in Bacillus subtilis
-
gene gltX1 and gltX2, expression in Escherichia coli temperature-sensitive mutant strain
-
gene gltX2, expression in Escherichia coli temperature-sensitive mutant strain
-
gene GluS, DNA and amino acid sequence determination and analysis
gene gluS, recombinant overexpression of C-terminally His-tagged GluRS in Escherichia coli
gene GUS1, phylogenetic analysis
overexpression in Escherichia coli
-
overexpression of GST-GluRS1
-
overexpression of GST-tagged isozyme GluRS1 in Escherichia coli strain Bl21(DE3), co-expression with Glu-tRNA reductase
-
overexpression of the enzyme in Escherichia coli with or without N-terminal His-tag
-
phylogenetic and expression analysis, the tnpA gene of the IS element and gene gltX are co-transcribed and their expression is transiently upregulated upon retrieval of the ammonium source irrespective of whether nitrate or no nitrogen source are available, structural elements of the promoter directing the expression of the tnpA-gltX operon are localized within the IS
Tolypothrix sp.
-
quantitative real-time PCR enzyme expression analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL
recombinant expression of enzyme mutant K236E/E328A as N-terminally His6-SUMO2-Gly-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) from plasmid DNA PLQ7619
-
TM1351, sequence comparisons, expression in Escherichia coli strain Rosetta2(DE3)
-