4.6.1.20: ribonuclease U2

This is an abbreviated version!
For detailed information about ribonuclease U2, go to the full flat file.

Reaction

Synonyms

EC 3.1.27.4, Pleospora RNase, Purine specific endoribonuclease, Purine-specific ribonuclease, Purine-specific RNase, Ribonuclease (purine), ribonuclease U2, ribonuclease U2A, ribonuclease U2B, Ribonuclease U3, RNase U2, RNase U2A, RNase U3, Trichoderma koningi RNase III, YoeB

ECTree

     4 Lyases

         4.6 Phosphorus-oxygen lyases

             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)

                4.6.1.20 ribonuclease U2

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Results	in table
2	AA Sequence
2	Application
1	CAS Registry Number
5	Cloned(Commentary)
5	Crystallization (Commentary)
7	General Information
15	Inhibitors
14	KM Value [mM]
5	Localization
1	Metals/Ions
8	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
28	Organism
4	pH Optimum
2	pI Value
7	Protein Variants
10	Purification (Commentary)
6	Reaction
4	Reaction Type
26	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
46	Substrates and Products (Substrate)
4	Subunits
22	Synonyms
1	Systematic Name
2	Temperature Stability [°C]
17	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 4.6.1.20 - ribonuclease U2

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SYSTEMATIC NAME

IUBMB Comments

[RNA]-purine 5'-hydroxy-ribonucleotide-3'-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3'-purine-nucleoside -2',3'-cyclophosphate-forming and hydrolysing)

The enzyme secreted by the fungus Ustilago sphaerogena cleaves at the 3'-phosphate group of purines, and catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending in Ap or Gp with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.