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4.6.1.20: ribonuclease U2

This is an abbreviated version!
For detailed information about ribonuclease U2, go to the full flat file.

Word Map on EC 4.6.1.20

Reaction

an [RNA fragment]-3'-adenosine-2',3'-cyclophosphate
+
H2O
=
an [RNA fragment]-3'-adenosine -3'-phosphate

Synonyms

EC 3.1.27.4, Pleospora RNase, Purine specific endoribonuclease, Purine-specific ribonuclease, Purine-specific RNase, Ribonuclease (purine), ribonuclease U2, ribonuclease U2A, ribonuclease U2B, Ribonuclease U3, RNase U2, RNase U2A, RNase U3, Trichoderma koningi RNase III, YoeB

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.20 ribonuclease U2

Reference

Reference on EC 4.6.1.20 - ribonuclease U2

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Uchida, T.; Egami, F.
Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
205-250
1971
Ustilago sphaerogena
-
Manually annotated by BRENDA team
Yasuda, T.; Inoue, Y.
Studies of catalysis by ribonuclease U2. Steady-state kinetics for transphosphorylation of oligonucleotide and synthetic substrates
Biochemistry
21
364-369
1982
Ustilago sphaerogena
Manually annotated by BRENDA team
Uchida, T.; Shibata, Y.
An affinity adsorbent, 5-adenylate-aminohexyl-sepharose. I. Purification and properties of two forms of RNase U2
J. Biochem.
90
463-471
1981
Ustilago sphaerogena
Manually annotated by BRENDA team
Minato, S.; Hirai, A.
Characterization of Ustilago Ribonuclease U2. Effects of chemical modification at glutamic acid-61 and cystine-1 and of organic solvents on the enzymatic activity
J. Biochem.
85
327-334
1979
Ustilago sphaerogena
Manually annotated by BRENDA team
Uchida, T.; Machida, C.
The specificity for dinucleoside monophosphates of RNase U2 and urea-treated RNase U2
Nucleic Acids Res.
1 Suppl 2
409-413
1978
Ustilago sphaerogena
-
Manually annotated by BRENDA team
Sato, S.; Uchida, T.
Ethoxyformylation of ribonuclease U2 form Ustilago sphaerogena
J. Biochem.
77
795-800
1975
Ustilago sphaerogena
Manually annotated by BRENDA team
Sato, S.; Uchida, T.
On the interaction of ribonuclease U-2 and substrate analogues
Biochim. Biophys. Acta
383
168-177
1975
Ustilago sphaerogena
Manually annotated by BRENDA team
Kioke, T.; Uchida, T.; Egami, F.
Synthesis of adenylyl-(3,5)-nucleosides, adenylyl-(3,5)-guanosine 2,3-cyclic phosphate, and oligoadenylic acids by ribonuclease U2
J. Biochem.
69
111-117
1971
Ustilago sphaerogena
Manually annotated by BRENDA team
Glitz, D.G.; Dekker, C.A.
A ribonuclease from Ustilago sphaerogena. I. Purification and properties of the enzyme
Biochemistry
3
1391-1399
1964
Ustilago sphaerogena
Manually annotated by BRENDA team
Glitz, D.G.; Dekker, C.A.
A ribonuclease from Ustilago sphaerogena. II. Specificity of the enzyme
Biochemistry
3
1399-1406
1964
Ustilago sphaerogena
Manually annotated by BRENDA team
Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Martinez del Pozo, A.; Gavilanes, J.G.
Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein
FEMS Microbiol. Lett.
189
165-169
2000
Ustilago sphaerogena (P00654), Ustilago sphaerogena, Ustilago sphaerogena CBS 534.71 (P00654)
Manually annotated by BRENDA team
Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Lacadena, J.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Del Pozo, A.M.; Gavilanes, J.G.
RNase U2 and alpha-sarcin: a study of relationships
Methods Enzymol.
341
335-351
2001
Ustilago sphaerogena (P00654)
Manually annotated by BRENDA team
Garcia-Ortega, L.; De los Rios, V.; Martinez-Ruiz, A.; Onaderra, M.; Lacadena, J.; Martinez del Pozo, A.; Gavilanes, J.G.
Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2
Electrophoresis
26
3407-3413
2005
Ustilago sphaerogena (P00654)
Manually annotated by BRENDA team
Kamada, K.; Hanaoka, F.
Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin
Mol. Cell
19
497-509
2005
Escherichia coli
Manually annotated by BRENDA team
Alvarez-Garcia, E.; Garcia-Ortega, L.; De los Rios, V.; Gavilanes, J.G.; Martinez-del-Pozo, A.
Influence of key residues on the heterologous extracellular production of fungal ribonuclease U2 in the yeast Pichia pastoris
Protein Expr. Purif.
65
223-229
2009
Ustilago sphaerogena (P00654), Ustilago sphaerogena
Manually annotated by BRENDA team
Chapman, E.G.; DeRose, V.J.
Enzymatic processing of platinated RNAs
J. Am. Chem. Soc.
132
1946-1952
2010
synthetic construct
Manually annotated by BRENDA team
Noguchi, S.
Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate
Acta Crystallogr. Sect. D
66
843-849
2010
Ustilago sphaerogena
Manually annotated by BRENDA team
Nabavi, S.; Nazar, R.N.
Cleavage-induced termination in U2 snRNA gene expression
Biochem. Biophys. Res. Commun.
393
461-465
2010
Schizosaccharomyces pombe, Schizosaccharomyces pombe GP969
Manually annotated by BRENDA team
Noguchi, S.
Structural changes induced by the deamidation and isomerization of asparagine revealed by the crystal structure of Ustilago sphaerogena ribonuclease U2B
Biopolymers
93
1003-1010
2010
Ustilago sphaerogena (P00654), Ustilago sphaerogena
Manually annotated by BRENDA team
Nabavi, S.; Nazar, R.N.
Pac1 endonuclease and Dhp1p 5'-3' exonuclease are required for U3 snoRNA termination in Schizosaccharomyces pombe
FEBS Lett.
584
3436-3441
2010
Schizosaccharomyces pombe, Schizosaccharomyces pombe GP969
Manually annotated by BRENDA team
Noguchi, S.
Conformational variation revealed by the crystal structure of RNase U2A complexed with Ca ion and 2'-adenylic acid at 1.03 A resolution
Protein Pept. Lett.
17
1559-1561
2010
Ustilago sphaerogena (P00654), Ustilago sphaerogena
Manually annotated by BRENDA team
Houser, W.M.; Butterer, A.; Addepalli, B.; Limbach, P.A.
Combining recombinant ribonuclease U2 and protein phosphatase for RNA modification mapping by liquid chromatography-mass spectrometry
Anal. Biochem.
478
52-58
2015
synthetic construct (KP779641)
Manually annotated by BRENDA team
Solivio, B.; Yu, N.; Addepalli, B.; Limbach, P.A.
Improving RNA modification mapping sequence coverage by LC-MS through a nonspecific RNase U2-E49A mutant
Anal. Chim. Acta
1036
73-79
2018
Ustilago sphaerogena (P00654)
Manually annotated by BRENDA team