4.6.1.20: ribonuclease U2
This is an abbreviated version!
For detailed information about ribonuclease U2, go to the full flat file.
Word Map on EC 4.6.1.20
-
4.6.1.20
-
sphaerogena
-
ustilago
-
main-chain
-
deamidation
-
succinimide
-
alpha-sarcin
-
trinucleotides
-
3'-terminal
-
isoaspartate
-
analysis
- 4.6.1.20
- sphaerogena
- ustilago
-
main-chain
-
deamidation
- succinimide
- alpha-sarcin
- trinucleotides
-
3'-terminal
-
isoaspartate
- analysis
Reaction
Synonyms
EC 3.1.27.4, Pleospora RNase, Purine specific endoribonuclease, Purine-specific ribonuclease, Purine-specific RNase, Ribonuclease (purine), ribonuclease U2, ribonuclease U2A, ribonuclease U2B, Ribonuclease U3, RNase U2, RNase U2A, RNase U3, Trichoderma koningi RNase III, YoeB
ECTree
Advanced search results
General Information
General Information on EC 4.6.1.20 - ribonuclease U2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
physiological function
additional information
-
the endonuclease cleavage of many small nuclear RNAs is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Reversed torpedoes model for the termination and maturation of the U2 snRNA, the Pac1 endonuclease cleavage provides entry sites for the 3' and 5' exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other
physiological function
-
the endonuclease cleavage of many small nuclear RNAs is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Reversed torpedoes model for the termination and maturation of the U2 snRNA, the Pac1 endonuclease cleavage provides entry sites for the 3' and 5' exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other
-
Asn32 of RNase U2A rapidly deaminates and isomerizes in alkaline conditions, conformational mechanism, overview. In solution, the region around Asn32-Gly33 is likely to be in equilibrium between multiple conformers with deamination of Asn32 proceeding when the region adopts an extended conformation
additional information
-
Asn32 of RNase U2A rapidly deaminates and isomerizes in alkaline conditions, conformational mechanism, overview. In solution, the region around Asn32-Gly33 is likely to be in equilibrium between multiple conformers with deamination of Asn32 proceeding when the region adopts an extended conformation
additional information
-
cleavage in the U3 snoRNA transcripts of Schizosaccharomyces pombe can induce transcript termination, the degree of cleavage correlates closely with both RNA maturation and transcript termination. the RNase III-like endonuclease, Pac1, and the nuclear 50-exonuclease, Dhp1p, are essential for RNA production and transcript termination, supporting a reversed torpedoes model in which the endonuclease cut allows 5'- and 3'-exonuclease activities access to the transcript, leading simultaneously to transcript termination in one direction and RNA maturation in the other
additional information
formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure, overview. IsoAsp32 protrudes from the surface of the protein, and the abnormal beta-peptide bond in the main-chain and alpha-carboxylate in the side-chain is fully exposed. The structure suggests that the deamidation of the Asn and the isoAsp formation in proteins could confer immunogenicity
additional information
-
formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure, overview. IsoAsp32 protrudes from the surface of the protein, and the abnormal beta-peptide bond in the main-chain and alpha-carboxylate in the side-chain is fully exposed. The structure suggests that the deamidation of the Asn and the isoAsp formation in proteins could confer immunogenicity
additional information
-
mechanism of isoaspartate formation within the Asp45-Glu46 sequence of ribonuclease U2, overview. In the succinimide-formation reaction water 219 receives a proton from the N atom of Glu46 as a general base and waters 190 and 220 stabilize the tetrahedral intermediate, and in the succinimide-hydrolysis reaction water 219 provides a proton for the N atom of Glu46 as a general acid
additional information
-
cleavage in the U3 snoRNA transcripts of Schizosaccharomyces pombe can induce transcript termination, the degree of cleavage correlates closely with both RNA maturation and transcript termination. the RNase III-like endonuclease, Pac1, and the nuclear 50-exonuclease, Dhp1p, are essential for RNA production and transcript termination, supporting a reversed torpedoes model in which the endonuclease cut allows 5'- and 3'-exonuclease activities access to the transcript, leading simultaneously to transcript termination in one direction and RNA maturation in the other
-