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4.6.1.20: ribonuclease U2

This is an abbreviated version!
For detailed information about ribonuclease U2, go to the full flat file.

Word Map on EC 4.6.1.20

Reaction

an [RNA fragment]-3'-adenosine-2',3'-cyclophosphate
+
H2O
=
an [RNA fragment]-3'-adenosine -3'-phosphate

Synonyms

EC 3.1.27.4, Pleospora RNase, Purine specific endoribonuclease, Purine-specific ribonuclease, Purine-specific RNase, Ribonuclease (purine), ribonuclease U2, ribonuclease U2A, ribonuclease U2B, Ribonuclease U3, RNase U2, RNase U2A, RNase U3, Trichoderma koningi RNase III, YoeB

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.20 ribonuclease U2

Crystallization

Crystallization on EC 4.6.1.20 - ribonuclease U2

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterotrimeric complex of (YefM)2-YoeB antitoxin-toxin pair and free YoeB structure. Conformational rearrangement of YoeB RNase catalytic site upon binding of YefM
-
isoAsp containing ribonuclease U2B, hanging drop vapor diffusion method, 0.004 ml of 10 mg/ml protein in 50 mM sodium citrate, pH 4.5, containing 50 mM sodium chloride, are mixed with 0.004 ml of reservoir solution containing 0.84 M sodium dihydrogen phosphate and 1.26 M dipotassium hydrogen phosphate, pH 6.9, equilibration against 0.4 ml reservoir solution, 20°C, 2 days, X-ray diffraction structure determination and analysis at 1.32 A resolution
ribonuclease U2 complexed with adenosine 3'-monophosphate, hanging drop vapour diffusion method, 0.005 ml of protein solution containing 20 mg/ml RNase U2A and 8 mM adenosine 3'-monophosphate, pH 4.5, is mixed with 0.005 ml of reservoir solution containing 12.5% w/w PEG 8000, 200 mM calcium acetate, 100 mM sodium cacodylate, pH 3.75, 12.5% w/w PEG 8000, equilibration against 0.4 ml reservoir solution, 20°C, 3 days, X-ray diffraction structure determination and analysis at 0.96-0.99 A resolution
-
RNase U2A in complex with 2'-adenylic acid and Ca2+ ions, X-ray diffraction structure determination and analysis at 1.03 A resolution