4.6.1.20: ribonuclease U2
This is an abbreviated version!
For detailed information about ribonuclease U2, go to the full flat file.
Word Map on EC 4.6.1.20
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4.6.1.20
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sphaerogena
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ustilago
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main-chain
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deamidation
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succinimide
-
alpha-sarcin
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trinucleotides
-
3'-terminal
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isoaspartate
-
analysis
- 4.6.1.20
- sphaerogena
- ustilago
-
main-chain
-
deamidation
- succinimide
- alpha-sarcin
- trinucleotides
-
3'-terminal
-
isoaspartate
- analysis
Reaction
Synonyms
EC 3.1.27.4, Pleospora RNase, Purine specific endoribonuclease, Purine-specific ribonuclease, Purine-specific RNase, Ribonuclease (purine), ribonuclease U2, ribonuclease U2A, ribonuclease U2B, Ribonuclease U3, RNase U2, RNase U2A, RNase U3, Trichoderma koningi RNase III, YoeB
ECTree
4.6.1.20 ribonuclease U2Advanced search results
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results (Substrates Products
Substrates Products on EC 4.6.1.20 - ribonuclease U2
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5'-(U)6-GA-(U)5-3' RNA + H2O
?
-
digestion of the platinated 5'-(U)6-GA-(U)5-3' RNA shows a reaction product corresponding to [UUUUUUGA + Pt(NH3)2 + H],+ depicting RNase cleavage 3' to an adenosine predicted to be involved in a cisplatin diadduct. The platinated G*A* RNA adduct is uniquely recognized and processed by RNase U2, whereas other purine-Pt(II) adducts are not. The basis for this selectivity may arise from the RNase's known A - G preference, allowing the endonuclease in some cases to partially recognize the 3' platinated A despite platinum modification
-
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
RNA + H2O
?
-
-
-
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
-
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
-
-
?
additional information
?
-
-
YoeB toxin has in vitro Rnase activity that preferentially cleaves at the 3-end of purine ribonucleotides
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-
?
additional information
?
-
-
specifically recognizes purine ribonucleotides and catalyzes cleavage of the 3' phosphodiester bond attached to a G or an A. For platinated sequences containing XY = GG, AG, and GU, RNase U2 cleavage is not observed, being blocked at the purine nucleotides predicted to be involved in platinum binding
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-
?
