4.2.1.30: glycerol dehydratase

This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.

Reaction

Synonyms

B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.30 glycerol dehydratase

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Results	in table
43	Activating Compound
411	AA Sequence
22	Application
1	CAS Registry Number
27	Cloned(Commentary)
63	Cofactor
5	Crystallization (Commentary)
13	General Information
4	General Stability
48	Inhibitors
9	kcat/KM [mM/s]
98	KM Value [mM]
32	Metals/Ions
49	Molecular Weight [Da]
78	Natural Substrates/ Products (Substrates)
176	Organism
2	Oxidation Stability
3	Pathway
12	PDB ID
15	pH Optimum
3	pH Range
4	pH Stability
38	Protein Variants
17	Purification (Commentary)
14	Reaction
1	Reaction Type
117	Reference
27	Specific Activity [micromol/min/mg]
3	Storage Stability
222	Substrates and Products (Substrate)
32	Subunits
66	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
2	Temperature Range [°C]
13	Temperature Stability [°C]
12	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 4.2.1.30 - glycerol dehydratase

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

16000

3 entries

161000

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

16101

Klebsiella pneumoniae

Q59475; A6NA24; A6NA25

x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis

705190

16121

Citrobacter freundii

-

alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid

33835, 33838

16722

Clostridium pasteurianum

-

alpha2,beta2,gamma2, 2 * 60813 + 2 * 19549 + 2 * 16722, calculation from sequence of amino acid

33838

17700

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

188000

Klebsiella pneumoniae

-

sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin

33819, 33824

189000

2 entries

190000

Clostridium pasteurianum

-

non-denaturing PAGE

33837

19549

Clostridium pasteurianum

-

alpha2,beta2,gamma2, 2 * 60813 + 2 * 19549 + 2 * 16722, calculation from sequence of amino acid

33838

198000

Citrobacter freundii

-

gel filtration, native enzyme

33835

200000

2 entries

21322

Klebsiella pneumoniae

Q59475; A6NA24; A6NA25

x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis

705190

21355

Klebsiella pneumoniae

-

2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid

33834

21487

Citrobacter freundii

-

alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid

33835, 33838

22000

5 entries

220000

Klebsiella pneumoniae

-

native PAGE, 2 bands: 200000 Da and 220000 Da

651133

229000

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

40900

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

42200

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

52000

Limosilactobacillus reuteri

-

4 * 52000, SDS-PAGE

33833

60433

Citrobacter freundii

-

alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid

33835, 33838

60659

Klebsiella pneumoniae

-

2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid

33834

60700

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

60702

Klebsiella pneumoniae

Q59475; A6NA24; A6NA25

x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis

705190

60813

Clostridium pasteurianum

-

alpha2,beta2,gamma2, 2 * 60813 + 2 * 19549 + 2 * 16722, calculation from sequence of amino acid

33838

61000

3 entries

62000

Klebsiella pneumoniae

A8CIU9

2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE

729463

83800

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

85100

Klebsiella pneumoniae

-

x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE

702830

90000

Klebsiella pneumoniae

-

gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits

33823, 33824

16000

Klebsiella pneumoniae

-

gamma-subunit, SDS-PAGE

651133, 651642

16000

Citrobacter freundii

A8CH84; A8CH91; A8CH95

x * 61000 + x * 22000 + x * 16000, SDS-PAGE

725951

16000

Klebsiella pneumoniae

A8CIU9

2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE

729463

189000

Klebsiella pneumoniae

-

gel filtration in the presence of 50 mM ethanolamine-HCl buffer, pH 8.6, subunit B

33824

189000

Klebsiella pneumoniae

-

gel filtration, in the presence of ethanolamine buffer, subunit B

33823

200000

Limosilactobacillus reuteri

-

gel filtration

33833

200000

Klebsiella pneumoniae

-

native PAGE, 2 bands: 200000 Da and 220000 Da

651133

22000

Klebsiella pneumoniae

-

gel filtration, subunit A, 20 mM potassium phosphate, pH 7.7, subunit A dissociates into sub-subunits of 11000 Da

33824

22000

Klebsiella pneumoniae

-

beta-subunit, SDS-PAGE

651133, 651642

22000

Klebsiella pneumoniae

-

1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE

33834

22000

Citrobacter freundii

A8CH84; A8CH91; A8CH95

x * 61000 + x * 22000 + x * 16000, SDS-PAGE

725951

22000

Klebsiella pneumoniae

A8CIU9

2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE

729463

61000

Klebsiella pneumoniae

-

alpha-subunit, SDS-PAGE

651133, 651642

61000

Klebsiella pneumoniae

-

1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE

33834

61000

Citrobacter freundii

A8CH84; A8CH91; A8CH95

x * 61000 + x * 22000 + x * 16000, SDS-PAGE

725951