4.2.1.30: glycerol dehydratase
This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.
Word Map on EC 4.2.1.30
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4.2.1.30
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pneumoniae
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klebsiella
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1,3-propanediol
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diol
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3-hydroxypropionaldehyde
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3-hydroxypropionic
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butyricum
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adenosylcobalamin-dependent
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adenosylcobalamin
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aerobacter
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synthesis
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freundii
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reactivase
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pdors
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2,3-butanediol
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pasteurianum
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1,2-ethanediol
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poly3-hydroxypropionate
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reuterin
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adocbl
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coenzyme-b12-dependent
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analysis
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biotechnology
- 4.2.1.30
- pneumoniae
- klebsiella
- 1,3-propanediol
- diol
- 3-hydroxypropionaldehyde
-
3-hydroxypropionic
- butyricum
-
adenosylcobalamin-dependent
- adenosylcobalamin
-
aerobacter
- synthesis
- freundii
-
reactivase
-
pdors
- 2,3-butanediol
- pasteurianum
- 1,2-ethanediol
-
poly3-hydroxypropionate
-
reuterin
-
adocbl
-
coenzyme-b12-dependent
- analysis
- biotechnology
Reaction
Synonyms
B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase
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Purification
Purification on EC 4.2.1.30 - glycerol dehydratase
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by Ni-nitrilotriacetate affinity chromatography followed by gel filtration
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homogeneity, enzyme is inactive after purification, but can be reactivated with glycerol dehydratase-activating enzyme under anaerobic conditions
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Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration
Ni-nitrilotriacetate agarose affinity column chromatography and Sephacryl S-300 gel filtration
Ni-NTA agarose column chromatography and Sephacryl S-300 gel filtration
A8CH84; A8CH91; A8CH95
recombinant His-tagged GDHt 2.11fold by nickel affinity chromatography and ultrafiltration
Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration