Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL10-Gold cells | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
pentane-1,2-diol | mutant enzyme KpG525, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
0.2 | - |
pentane-1,2-diol | mutant enzyme KpG60, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
0.24 | - |
pentane-1,2-diol | wild type enzyme, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
0.6 | - |
glycerol | wild type enzyme, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
0.6 | - |
glycerol | mutant enzyme KpG525, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
0.7 | - |
glycerol | mutant enzyme KpG60, at pH 6.0 and 37°C | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE | Klebsiella pneumoniae |
22000 | - |
2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE | Klebsiella pneumoniae |
62000 | - |
2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | Klebsiella pneumoniae | - |
3-hydroxypropanal + H2O | - |
? | |
glycerol | Klebsiella pneumoniae 10018 | - |
3-hydroxypropanal + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | A8CIU9 | large subunit | - |
Klebsiella pneumoniae 10018 | A8CIU9 | large subunit | - |
Purification (Comment) | Organism |
---|---|
Ni-nitrilotriacetate agarose affinity column chromatography and Sephacryl S-300 gel filtration | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae 10018 | 3-hydroxypropanal + H2O | - |
? | |
pentane-1,2-diol | - |
Klebsiella pneumoniae | ? | - |
? | |
pentane-1,2-diol | - |
Klebsiella pneumoniae 10018 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | 2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
GDHt | - |
Klebsiella pneumoniae |
KpG | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
pentane-1,2-diol | wild type enzyme, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
250 | - |
pentane-1,2-diol | mutant enzyme KpG60, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
310 | - |
pentane-1,2-diol | mutant enzyme KpG525, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
430 | - |
glycerol | wild type enzyme, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
460 | - |
glycerol | mutant enzyme KpG60, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
890 | - |
glycerol | mutant enzyme KpG525, at pH 6.0 and 37°C | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Klebsiella pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
660 | - |
glycerol | mutant enzyme KpG60, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
710 | - |
glycerol | wild type enzyme, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
840 | - |
pentane-1,2-diol | wild type enzyme, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
1200 | - |
pentane-1,2-diol | mutant enzyme KpG60, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
1400 | - |
glycerol | mutant enzyme KpG525, at pH 6.0 and 37°C | Klebsiella pneumoniae | |
1500 | - |
pentane-1,2-diol | mutant enzyme KpG525, at pH 6.0 and 37°C | Klebsiella pneumoniae |