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Literature summary for 4.2.1.30 extracted from

  • Tobimatsu, T.; Azuma, M.; Matsubara, H.; Takatori, H.; Niida, T.; Nishimoto, K.; Satoh, H.; Hayashi, R.; Toraya, T.
    Cloning, sequencing, and high level expression of the genes encoding adenosylcobalamin-dependent glycerol dehydrase of Klebsiella pneumoniae (1996), J. Biol. Chem., 271, 22352-22357.
    View publication on PubMed
Search for more literature for 4.2.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21355
-
 2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid Klebsiella pneumoniae
22000
-
 1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE Klebsiella pneumoniae
60659
-
 2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid Klebsiella pneumoniae
61000
-
 1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
 ?
-
 ?
additional information Klebsiella pneumoniae enzyme production when grown anaerobically in a glycerol medium ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 ATCC 25955
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae ?
-
 ?
additional information enzyme production when grown anaerobically in a glycerol medium Klebsiella pneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
dimer !1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE Klebsiella pneumoniae
dimer 2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
adenosylcobalamin required Klebsiella pneumoniae